Background: Preeclampsia (PE) is a disorder that occurs in at least 5% of pregnancies and affects both the mother and the unborn baby. A dramatic increase of maternal serum inhibin A concentration in the second and third trimester of pregnancy is a common feature of PE and inhibin A measurement may add significant prognostic information for predicting PE in pregnant women. Design: We evaluated the presence and prevalence of gene polymorphisms for inhibin a subunit (INH alpha) in patients affected by PE (no.=50; study group), and in the general population (control group composed of 103 women and 42 men). Methods: DNA extraction, single strand conformation polymorphism analysis, DNA sequencing, restriction fragment length polymorphism analysis, and Fisher's exact test were used. Results: A 769G-A transition was found in INH alpha 1, but not in INH alpha 2 or INH alpha 3 fragment. This variant was found in 10/145 normal controls (7,6%), and in 1/50 preeclamptic patients (2%), without significant difference between the two groups (p=0.29). Conclusions: The prevalence of INH alpha gene variants is not increased in PE. Due to its frequency, the 769G -> A transition may be considered a polymorphism present in the general Italian population.
Ciarmela, P., Florio, P., Battistini, S., Grasso, D., Amato, T., Boschi, S., et al. (2005). Mutational analysis of the inhibin alpha gene in preeclamptic women. JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION, 28(1), 30-33 [10.1007/BF03345526].
Mutational analysis of the inhibin alpha gene in preeclamptic women
Florio, P.;Battistini, S.;
2005-01-01
Abstract
Background: Preeclampsia (PE) is a disorder that occurs in at least 5% of pregnancies and affects both the mother and the unborn baby. A dramatic increase of maternal serum inhibin A concentration in the second and third trimester of pregnancy is a common feature of PE and inhibin A measurement may add significant prognostic information for predicting PE in pregnant women. Design: We evaluated the presence and prevalence of gene polymorphisms for inhibin a subunit (INH alpha) in patients affected by PE (no.=50; study group), and in the general population (control group composed of 103 women and 42 men). Methods: DNA extraction, single strand conformation polymorphism analysis, DNA sequencing, restriction fragment length polymorphism analysis, and Fisher's exact test were used. Results: A 769G-A transition was found in INH alpha 1, but not in INH alpha 2 or INH alpha 3 fragment. This variant was found in 10/145 normal controls (7,6%), and in 1/50 preeclamptic patients (2%), without significant difference between the two groups (p=0.29). Conclusions: The prevalence of INH alpha gene variants is not increased in PE. Due to its frequency, the 769G -> A transition may be considered a polymorphism present in the general Italian population.File | Dimensione | Formato | |
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https://hdl.handle.net/11365/20403
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