Uricase catalyzes the oxidation of urate to form allantoin, carbon dioxide and hydrogen peroxide. In this article, we demonstrate for the first time the presence of a radical intermediate involved in the reaction mechanism. Such radical species was entrapped using 5-diethoxyphosphoryl-5-methyl-1-pyrroline N-oxide as spin trap and the relative adduct was detected by electron paramagnetic resonance (EPR) technique. A structure of such radical (5-hydroperoxy isourate) is proposed, through chemical results and density functional theory calculations of the EPR coupling constants.

Busi, E., Sinicropi, A., Terzuoli, L., Marinello, E., & Basosi, R. (2007). Identification and structural characterization of a transient Radical Species in the Uricase Reaction Mechanism. APPLIED MAGNETIC RESONANCE, 31(3-4), 471-482 [10.1007/BF03166597].

Identification and structural characterization of a transient Radical Species in the Uricase Reaction Mechanism

BUSI, ELENA;SINICROPI, ADALGISA;BASOSI, RICCARDO
2007

Abstract

Uricase catalyzes the oxidation of urate to form allantoin, carbon dioxide and hydrogen peroxide. In this article, we demonstrate for the first time the presence of a radical intermediate involved in the reaction mechanism. Such radical species was entrapped using 5-diethoxyphosphoryl-5-methyl-1-pyrroline N-oxide as spin trap and the relative adduct was detected by electron paramagnetic resonance (EPR) technique. A structure of such radical (5-hydroperoxy isourate) is proposed, through chemical results and density functional theory calculations of the EPR coupling constants.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11365/19477
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