Background & objectives: Environmental pollutants have detrimental effects on the reproductive function of human being and of animals. This study was aimed at evaluating the effects of tire debris, a fraction of particulate matter derived from tire wear, on motility and morphology of human spermatozoa. Methods: spermatozoa from ten healthy men were incubated for 4 hours with 10, 50 and 75 μg/ml of tire debris organic extract (TDOE). Sperm motility and morphology were evaluated according to WHO guidelines; the presence of apoptosis and necrosis was assessed by the AnnexinV/Propidium iodide assay. Ultrastructure of sperm organelles was observed by transmission electron microscopy. Results: incubation with 10 μg/ml of TDOE did not affect sperm quality, except for the value of apoptosis (median: 8% vs. 6%; P<0.01). Sperm motility and sperm morphology percentages (P<0.01)decreased when sperm were treated with 50 and 75 μg/ml TDOE and the percentage of apoptotic and necrotic sperm (P<0.01) increased concomitantly. At the same concentrations TEM analysis showed an increased percentage of reacted and swollen acrosomes, disrupted chromatin and altered axonemes. Interpretation & conclusions: the organic part of tire debris is potentially harmful to spermatozoa, particularly at a concentration of 50-75 μg/ml, inhibiting motility and compromising morphological integrity. Nevertheless, these results cannot yet be extrapolated to the in vivo outcome due to the lack of studies focused on this issue. © Geminiani et al.; Licensee Bentham Open.

Geminiani, M., Moretti, E., Cetta, F., Castellini, C., Terzuoli, G., Mazzi, L., et al. (2011). In vitro effects of tire debris organic extracts on human spermatozoa. THE OPEN ANDROLOGY JOURNAL, 3, 8-13 [10.2174/1876827X01103010008].

In vitro effects of tire debris organic extracts on human spermatozoa

GEMINIANI, M.;MORETTI, E.;CETTA, F.;CASTELLINI, C.;TERZUOLI, G.;MAZZI, L.;COLLODEL, G.
2011-01-01

Abstract

Background & objectives: Environmental pollutants have detrimental effects on the reproductive function of human being and of animals. This study was aimed at evaluating the effects of tire debris, a fraction of particulate matter derived from tire wear, on motility and morphology of human spermatozoa. Methods: spermatozoa from ten healthy men were incubated for 4 hours with 10, 50 and 75 μg/ml of tire debris organic extract (TDOE). Sperm motility and morphology were evaluated according to WHO guidelines; the presence of apoptosis and necrosis was assessed by the AnnexinV/Propidium iodide assay. Ultrastructure of sperm organelles was observed by transmission electron microscopy. Results: incubation with 10 μg/ml of TDOE did not affect sperm quality, except for the value of apoptosis (median: 8% vs. 6%; P<0.01). Sperm motility and sperm morphology percentages (P<0.01)decreased when sperm were treated with 50 and 75 μg/ml TDOE and the percentage of apoptotic and necrotic sperm (P<0.01) increased concomitantly. At the same concentrations TEM analysis showed an increased percentage of reacted and swollen acrosomes, disrupted chromatin and altered axonemes. Interpretation & conclusions: the organic part of tire debris is potentially harmful to spermatozoa, particularly at a concentration of 50-75 μg/ml, inhibiting motility and compromising morphological integrity. Nevertheless, these results cannot yet be extrapolated to the in vivo outcome due to the lack of studies focused on this issue. © Geminiani et al.; Licensee Bentham Open.
Geminiani, M., Moretti, E., Cetta, F., Castellini, C., Terzuoli, G., Mazzi, L., et al. (2011). In vitro effects of tire debris organic extracts on human spermatozoa. THE OPEN ANDROLOGY JOURNAL, 3, 8-13 [10.2174/1876827X01103010008].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/19365
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