Iron is released in a free [desferrioxamine (DFO)-chelatable] form in mouse erythrocytes incubated with the oxidizing agents acrolein and phenylhydrazine or in erythrocytes drawn from allyl alcohol-intoxicated mice. The release is accompanied by peroxidation of membrane lipids when the cells are depleted of glutathione. Lipid peroxidation is always followed by the lysis of the cells. The release of iron is also accompanied by methemoglobin formation, but the extent of the release does not correlate with the level of methemoglobin production. The addition of DFO to the incubation mixture or the preincubation of the erythrocytes with DFO in millimolar concentrations completely prevents both lipid peroxidation and hemolysis while not significantly changing the level of iron release. Morphological studies carried out with scanning electron microscopy showed a number of alterations in the shape of the incubated erythrocytes, including echinocyte transformation and the appearance of codocyte, stomatocyte, and cnizocyte like forms. These alterations were more prominent with increasing lipid peroxidation and hemolysis, even if occurring in their absence. On the contrary, the appearance of pits and holes was strictly associated with lipid peroxidation and lysis. © 1994 Academic Press, Inc.
Ciccoli, L., Signorini, C., Alessandrini, C., Ferrali, M., Comporti, M. (1994). Iron release, lipid peroxidation, and morphological alterations of erythrocytes exposed to acrolein and phenylhydrazine. EXPERIMENTAL AND MOLECULAR PATHOLOGY, 60(2), 108-118 [10.1006/exmp.1994.1010].
Iron release, lipid peroxidation, and morphological alterations of erythrocytes exposed to acrolein and phenylhydrazine
CICCOLI, L.;SIGNORINI, C.;ALESSANDRINI, C.;FERRALI, M.;COMPORTI, M.
1994-01-01
Abstract
Iron is released in a free [desferrioxamine (DFO)-chelatable] form in mouse erythrocytes incubated with the oxidizing agents acrolein and phenylhydrazine or in erythrocytes drawn from allyl alcohol-intoxicated mice. The release is accompanied by peroxidation of membrane lipids when the cells are depleted of glutathione. Lipid peroxidation is always followed by the lysis of the cells. The release of iron is also accompanied by methemoglobin formation, but the extent of the release does not correlate with the level of methemoglobin production. The addition of DFO to the incubation mixture or the preincubation of the erythrocytes with DFO in millimolar concentrations completely prevents both lipid peroxidation and hemolysis while not significantly changing the level of iron release. Morphological studies carried out with scanning electron microscopy showed a number of alterations in the shape of the incubated erythrocytes, including echinocyte transformation and the appearance of codocyte, stomatocyte, and cnizocyte like forms. These alterations were more prominent with increasing lipid peroxidation and hemolysis, even if occurring in their absence. On the contrary, the appearance of pits and holes was strictly associated with lipid peroxidation and lysis. © 1994 Academic Press, Inc.File | Dimensione | Formato | |
---|---|---|---|
Exp Mol Pathol 1994.pdf
non disponibili
Tipologia:
Post-print
Licenza:
NON PUBBLICO - Accesso privato/ristretto
Dimensione
1.43 MB
Formato
Adobe PDF
|
1.43 MB | Adobe PDF | Visualizza/Apri Richiedi una copia |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
https://hdl.handle.net/11365/19287
Attenzione
Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo