The aim of the present study was to demonstrate induction of vitellogenin in the common carp (Cyprinus carpio) as a biomarker for monitoring freshwater ecosystems. Sexually undifferentiated specimens of common carp were treated experimentally with 17beta-estradiol and increasing doses of 4-nonylphenol and levels of plasma Vtg were measured in order to: 1) validate an ELISA assay for plasma Vtg in the common carp using the polyclonal rabbit anti-salmon Vtg antibody AA1 (Biosense, Norway); 2) check the sensitivity of carp juveniles in producing Vtg in response to estrogen stimulation. The group treated with 17beta-estradiol showed high induction (156%) with respect to controls, also groups treated with 4-nonylphenol showed induction of Vtg. The group treated with 100 mg kg(-1) b.wt showed an induction of 61%. A statistically significant correlation was found between dose and response. This preliminary study demonstrate a response to the ELISA assay for Vtg in the common carp using rabbit anti-salmon antibody AA1. With further evidence the tested biomarker might be proposed for large scale monitoring of estrogenic effects caused by pollution in urban and industrial effluents.

Casini, S., Fossi, M.C., Mori, G., Bjornstad, A. (2002). Vitellogenin induction in Cyprinus carpio treated with 17 beta-estradiol and 4-nonylphenol. ENVIRONMENTAL MONITORING AND ASSESSMENT, 75(3), 235-239 [10.1023/A:1014848100515].

Vitellogenin induction in Cyprinus carpio treated with 17 beta-estradiol and 4-nonylphenol

CASINI, S.;FOSSI, M. C.;
2002-01-01

Abstract

The aim of the present study was to demonstrate induction of vitellogenin in the common carp (Cyprinus carpio) as a biomarker for monitoring freshwater ecosystems. Sexually undifferentiated specimens of common carp were treated experimentally with 17beta-estradiol and increasing doses of 4-nonylphenol and levels of plasma Vtg were measured in order to: 1) validate an ELISA assay for plasma Vtg in the common carp using the polyclonal rabbit anti-salmon Vtg antibody AA1 (Biosense, Norway); 2) check the sensitivity of carp juveniles in producing Vtg in response to estrogen stimulation. The group treated with 17beta-estradiol showed high induction (156%) with respect to controls, also groups treated with 4-nonylphenol showed induction of Vtg. The group treated with 100 mg kg(-1) b.wt showed an induction of 61%. A statistically significant correlation was found between dose and response. This preliminary study demonstrate a response to the ELISA assay for Vtg in the common carp using rabbit anti-salmon antibody AA1. With further evidence the tested biomarker might be proposed for large scale monitoring of estrogenic effects caused by pollution in urban and industrial effluents.
2002
Casini, S., Fossi, M.C., Mori, G., Bjornstad, A. (2002). Vitellogenin induction in Cyprinus carpio treated with 17 beta-estradiol and 4-nonylphenol. ENVIRONMENTAL MONITORING AND ASSESSMENT, 75(3), 235-239 [10.1023/A:1014848100515].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/19206
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