Streptomyces sp A21 is a cellulolytic strain isolated from soil which was assigned to the genus Streptomyces on the basis of distinctive morphological features. A genomic library of A21 DNA has been constructed and transformed into Escherichia coli K-12 using a high-copy-number vector. One of the recombinant plasmids activates the cryptic bgl operon when inserted into appropriate strains. The complete sequence of the 1629-bp A21 DNA fragment has been determined. The analysis revealed the presence of an ORF whose putative product shows a high degree of similarity to RNA polymerase sigma factors; we therefore designated the gene psfS (Putative sigma factor, Streptomyces). Mapping of the 5' terminus of transcript by primer extension indicated that PsfS induces transcription initiation within the bgl promoter-silencer region.
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|Titolo:||A putative Streptomyces sigma factor can activate the expression of the cryptic operon bgl in Escherichia coli K-12.|
|Rivista:||MOLECULAR AND GENERAL GENETICS|
|Citazione:||Marri, L., Daly, S., Perito, B., Tamburini, E., & Mastromei, G. (1999). A putative Streptomyces sigma factor can activate the expression of the cryptic operon bgl in Escherichia coli K-12. MOLECULAR AND GENERAL GENETICS, 261(1), 201-207.|
|Appare nelle tipologie:||1.1 Articolo in rivista|