Psoriasis is a multisystemic inflammatory disorder mainly involving the skin and joints, whose etiopathogenesis is still not completely understood. An association with streptococcal throat infection has been suggested. We aim to investigate a correlation between IL-17A and IFN-gamma production by T cells infiltrating skin lesions and PASI in 313 patients with psoriasis, compared with that in 252 healthy controls. The phenotype of beta-hemolytic Streptococci-specific infiltrating T cells in skin lesions was evaluated and characterized for IFN-gamma, IL-4, and IL-17A production. In addition, PBMCs were tested by ELISpot for IFN-gamma and IL-17A after streptococcal antigen exposure. A total of 64 of 313 (20.4%) patients with psoriasis had throat streptococcal infection. Of the 3,868 skin-derived T-cell clones from psoriasis with streptococcal infection, 66% proliferated in response to beta-hemolytic Streptococci antigens. Most beta-hemolytic Streptococci-specific T cells displayed T helper 17 and T helper 1 phenotypes. The levels of IFN-gamma and IL-17A secreted by skin-infiltrating T cells of patients with psoriasis significantly correlated with PASI score. In beta-hemolytic Streptococci-positive patients, IFN-gamma and IL-17A production by peripheral blood T cells after stimulation with streptococcal antigens was quantified by ELISpot. The results obtained may suggest ELISpot as a useful diagnostic tool to identify patients with psoriasis that may deserve antibiotic treatment.
Della Bella, C., Corrà, A., Mantengoli, E., Galano, A., Benagiano, M., Bonciani, D., et al. (2023). Skin IL-17A and IFN-γ Production Correlate with Disease Severity in Patients with Psoriasis and Streptococcal Infection. JOURNAL OF INVESTIGATIVE DERMATOLOGY, 143(6), 925-932 [10.1016/j.jid.2022.10.025].
Skin IL-17A and IFN-γ Production Correlate with Disease Severity in Patients with Psoriasis and Streptococcal Infection
Della Bella, Chiara;Mantengoli, Elisabetta;Galano, Angelo;Aimo, Cristina;D'Elios, Mario Milco;
2023-01-01
Abstract
Psoriasis is a multisystemic inflammatory disorder mainly involving the skin and joints, whose etiopathogenesis is still not completely understood. An association with streptococcal throat infection has been suggested. We aim to investigate a correlation between IL-17A and IFN-gamma production by T cells infiltrating skin lesions and PASI in 313 patients with psoriasis, compared with that in 252 healthy controls. The phenotype of beta-hemolytic Streptococci-specific infiltrating T cells in skin lesions was evaluated and characterized for IFN-gamma, IL-4, and IL-17A production. In addition, PBMCs were tested by ELISpot for IFN-gamma and IL-17A after streptococcal antigen exposure. A total of 64 of 313 (20.4%) patients with psoriasis had throat streptococcal infection. Of the 3,868 skin-derived T-cell clones from psoriasis with streptococcal infection, 66% proliferated in response to beta-hemolytic Streptococci antigens. Most beta-hemolytic Streptococci-specific T cells displayed T helper 17 and T helper 1 phenotypes. The levels of IFN-gamma and IL-17A secreted by skin-infiltrating T cells of patients with psoriasis significantly correlated with PASI score. In beta-hemolytic Streptococci-positive patients, IFN-gamma and IL-17A production by peripheral blood T cells after stimulation with streptococcal antigens was quantified by ELISpot. The results obtained may suggest ELISpot as a useful diagnostic tool to identify patients with psoriasis that may deserve antibiotic treatment.File | Dimensione | Formato | |
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https://hdl.handle.net/11365/1277521