: Robust methods for manipulation of human B cells, isolated from healthy donors and patients with B cell disorders, has the potential to significantly accelerate B cell research. Our work describes a step-by-step protocol to perform electroporation-based screening of gene function in B cells through the use of Cas9 ribonuclecomplexes and in vitro produced mRNA.
Nardi, F., Pezzella, L., Drago, R., Di Rita, A., Simoncelli, M., Marotta, G., et al. (2022). Assessing gene function in human B cells: CRISPR/Cas9-based gene editing and mRNA-based gene expression in healthy and tumor cells. EUROPEAN JOURNAL OF IMMUNOLOGY, 52(8), 1362-1365 [10.1002/eji.202149784].
Assessing gene function in human B cells: CRISPR/Cas9-based gene editing and mRNA-based gene expression in healthy and tumor cells
Drago R.;Simoncelli M.;Gozzetti A.;Bocchia M.;Kabanova A.
2022-01-01
Abstract
: Robust methods for manipulation of human B cells, isolated from healthy donors and patients with B cell disorders, has the potential to significantly accelerate B cell research. Our work describes a step-by-step protocol to perform electroporation-based screening of gene function in B cells through the use of Cas9 ribonuclecomplexes and in vitro produced mRNA.
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https://hdl.handle.net/11365/1232117