Preferential Th1 profile of T helper cell responses in X-linked (Bruton's) agammaglobulinemia

X-linked agammaglobulinemia (XLA) is a primary immunodeficiency of the B-cell compartment caused by a defective gene encoding for the tyrosine kinase (btk) essential for B cell differentiation. Affected males undergo recurrent pyogenic infections and deficient immunoglobulin production. Peripheral blood T cells from 6 XLA patients and 6 matched healthy controls were stimulated with either PHA or tetanus toroid (TT) and T cell clones obtained were compared for their cytokine profile. In the series of PHA-induced or rr-specific CD4(+) T cell clones derived from XLA patients, the Th1 profile was predominant (63 and 65%, respectively). Upon stimulation with TT, the proportion of activated T cells from XLA that expressed the IFN-gamma -associated LAG-3 activation molecule was higher than in control T cells (51 vs. 25%), whereas the expression of the IL-4-associated CD30 molecule was lower (5 vs. 21%). in a cohort of 31 XLA patients, plasma levels of soluble (s)LAG-3 and sCD30, chosen as indirect indicators of the Th1/Th2 activity in vivo, were significantly higher and lower, respectively, than those measured in 31 healthy controls. Likewise, plasma levels of interferon-inducible protein 10 and of macrophage-derived chemokine in XLA patients were significantly higher and lower, respectively,than in healthy controls.