A multivariate strategy was used to optimize an adsorptive stripping voltammetric method for the determination of the antiulcer drug omeprazole. A 3/4 matrix was used for the variable screening while a central composite design was chosen in the subsequent step to evaluate the response surfaces. Simultaneous optimization of the response peak height (h(p)) and peak half width w(1/2)), the latter being a peak shape measure, was achieved. The factors accumulation time, pulse amplitude, scan rate and stirring rate were all found to be statistically significant for the response h(p), while for the response w(1/2) only the stirring rate was found to be significant. The optimized method shows a good linearity between peak height and analyte concentration in the concentration range from 8.33 x 10-9 M to 1.42 x 10- 7 M with a LOD of 6.5 x 10-9 M. The mean recovery of omeprazole in capsules was 101.9% with a SD of 2.04 (RSD = 200).
Pinzauti, S., Gratteri, P., Furlanetto, S., Mura, P., Dreassi, E., Phan-Tan-Luu, R. (1996). Experimental design in the development of voltammetric method for the assay of omeprazole. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 14(8-10), 881-889 [10.1016/0731-7085(95)01683-X].
Experimental design in the development of voltammetric method for the assay of omeprazole
Dreassi E.;
1996-01-01
Abstract
A multivariate strategy was used to optimize an adsorptive stripping voltammetric method for the determination of the antiulcer drug omeprazole. A 3/4 matrix was used for the variable screening while a central composite design was chosen in the subsequent step to evaluate the response surfaces. Simultaneous optimization of the response peak height (h(p)) and peak half width w(1/2)), the latter being a peak shape measure, was achieved. The factors accumulation time, pulse amplitude, scan rate and stirring rate were all found to be statistically significant for the response h(p), while for the response w(1/2) only the stirring rate was found to be significant. The optimized method shows a good linearity between peak height and analyte concentration in the concentration range from 8.33 x 10-9 M to 1.42 x 10- 7 M with a LOD of 6.5 x 10-9 M. The mean recovery of omeprazole in capsules was 101.9% with a SD of 2.04 (RSD = 200).
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
https://hdl.handle.net/11365/11903
Attenzione
Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo