CD4 engagement triggers an early signaling cascade which initiates late events such as transcription factor activation. The outcome of CD4 engagement is T-cell commitment to alternative, dramatically different fates, such as activation and apoptosis. We have tested a panel of anti-CD4 mAbs specific for different CD4 epitopes, as well as HIV-1 gp120, for the capacity to activate crucial early events such as enhancement of p561ck kinase activity and Shc phosphoryation. The same CD4 epitopes were characterized for their capacity both to deliver a gene activating signal and to program T-cells to activation dependent death. No correlation could be found between capacity of specific CD4 epitopes to deliver a gene activating signal and capacity to prime T-cells to apoptotis, suggesting that gene activating and proapoptotic potential are independent functions of CD4 epitopes. Furthermore, while triggering of the calcium pathway appears critical in NF-AT activation, optimal p561ck activation and Shc phosphorylation might be required for initiation of the apoptotic pathway.
|Titolo:||Gene activating and proapoptotic potential are independent properties of different CD4 epitopes|
|Citazione:||Milia, E., DI SOMMA, M.M., Majolini, M.B., Ulivieri, C., Somma, C., Piccolella, E., et al. (1997). Gene activating and proapoptotic potential are independent properties of different CD4 epitopes. MOLECULAR IMMUNOLOGY, 34(4), 287-296.|
|Appare nelle tipologie:||1.1 Articolo in rivista|
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