Characterizing the impact of the vaccination schedule on the induction of B and T cell immune responses is critical for improving vaccine immunogenicity. Here we compare the effect of a short (4 weeks) or a long (18 weeks) interval between priming and boosting in mice, using a model vaccine formulation based on the chimeric tuberculosis vaccine antigen H56 combined with alum. While no significant difference was observed in serum antigen-specific IgG response and the induction of antigen-specific T follicular helper cells into draining lymph nodes after the two immunization schedules, a longer interval between priming and boosting elicited a higher number of germinal center-B cells and H56-specific antibody-secreting cells and modulated the effector function of reactivated CD4+ T cells. These data show that the scheduling of the booster immunization could affect the immune response elicited by vaccination modulating and improving the immunogenicity of the vaccine. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Pettini, E., Pastore, G., Fiorino, F., Medaglini, D., Ciabattini, A. (2021). Short or Long Interval between Priming and Boosting: Does It Impact on the Vaccine Immunogenicity?. VACCINES, 9(3), 1-14 [10.3390/vaccines9030289].
Short or Long Interval between Priming and Boosting: Does It Impact on the Vaccine Immunogenicity?
Pettini, Elena;Pastore, Gabiria;Fiorino, Fabio;Medaglini, Donata;Ciabattini, Annalisa
2021-01-01
Abstract
Characterizing the impact of the vaccination schedule on the induction of B and T cell immune responses is critical for improving vaccine immunogenicity. Here we compare the effect of a short (4 weeks) or a long (18 weeks) interval between priming and boosting in mice, using a model vaccine formulation based on the chimeric tuberculosis vaccine antigen H56 combined with alum. While no significant difference was observed in serum antigen-specific IgG response and the induction of antigen-specific T follicular helper cells into draining lymph nodes after the two immunization schedules, a longer interval between priming and boosting elicited a higher number of germinal center-B cells and H56-specific antibody-secreting cells and modulated the effector function of reactivated CD4+ T cells. These data show that the scheduling of the booster immunization could affect the immune response elicited by vaccination modulating and improving the immunogenicity of the vaccine. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.File | Dimensione | Formato | |
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https://hdl.handle.net/11365/1135962