Keratoconus (KC) is the most common degenerative corneal disease and no single biomarker for KC has been discovered. Its causes have not yet been clarified and this work aims to be a contribution to the deepening of the knowledge of this disease and a preliminary data to the evaluation of the possibility of the use of copper (Cu) concentration in the tear fluid as a specific marker. A tear fluid sampling and Cu determination by spectrometric atomic absorption method was optimized to determine Cu levels in the tear fluid of patients with KC compared to that of healthy patients. Results demonstrate that in the KC subjects (n=6) the concentration of Cu ions was 325.5 ± 110.7 ng/ml, while in the control group was 141.3 ± 71.1 ng/ml. A significant increase in Cu ion levels in the tear fluid was observed in the KC group compared to the control group (p value < 0.001).

Corbini, G., Dreassi, E., Chiasserini, L., Girolamo, M.M., Mellace, P. (2021). Determination of copper by AAS in tear fluid of patients with keratoconus. ANALYTICAL BIOCHEMISTRY, 623 [10.1016/j.ab.2021.114174].

Determination of copper by AAS in tear fluid of patients with keratoconus

Gianfranco Corbini
Conceptualization
;
Elena Dreassi
Data Curation
;
Luisa Chiasserini
Formal Analysis
;
Matteo Maria Girolamo
Formal Analysis
;
Pierfrancesco Mellace
Conceptualization
2021-01-01

Abstract

Keratoconus (KC) is the most common degenerative corneal disease and no single biomarker for KC has been discovered. Its causes have not yet been clarified and this work aims to be a contribution to the deepening of the knowledge of this disease and a preliminary data to the evaluation of the possibility of the use of copper (Cu) concentration in the tear fluid as a specific marker. A tear fluid sampling and Cu determination by spectrometric atomic absorption method was optimized to determine Cu levels in the tear fluid of patients with KC compared to that of healthy patients. Results demonstrate that in the KC subjects (n=6) the concentration of Cu ions was 325.5 ± 110.7 ng/ml, while in the control group was 141.3 ± 71.1 ng/ml. A significant increase in Cu ion levels in the tear fluid was observed in the KC group compared to the control group (p value < 0.001).
2021
Corbini, G., Dreassi, E., Chiasserini, L., Girolamo, M.M., Mellace, P. (2021). Determination of copper by AAS in tear fluid of patients with keratoconus. ANALYTICAL BIOCHEMISTRY, 623 [10.1016/j.ab.2021.114174].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/1134946