The RT-PCR analysis of RNA from progenitor and differentiated primary rat oligodendrocytes, and from the oligodendrocyte CG-4 cell line, shows the presence of the IL-1β mRNA, the type I IL-1 receptor and the IL-1 receptor accessory protein in these cells. In situ hybridization of a rat IL-1β probe to primary progenitor and differentiated rat oligodendrocytes results in a positive signal. The double hybridization of the IL-1β probe, together with an oligodendrocyte-specific differentiation marker, to sections of postnatal rat brain at different stages of differentiation is also positive. The double immune-labelling technique utilized indicates coincidence of the signals on the brain slices. The results show that IL-1β mRNA is constitutively expressed in rat brain oligodendrocytes from 1 day after birth onward. In agreement with this observation, CG-4 cells, primary progenitor and differentiated rat oligodendrocytes are positively stained by antibodies against IL-1β. Postnatal brain slices from 1 and 4 day old and adult rats, labelled with a double immunofluorescence technique, are also stained by antibodies against IL-1β. This signal coincides with that of antibodies against oligodendrocyte-specific surface markers. We conclude that IL-1β is constitutively expressed in rat brain progenitor and differentiated oligodendrocytes.

Blasi, F., Riccio, M., Brogi, A., Strazza, M., Taddei, M.L., Romagnoli, S., et al. (1999). Constitutive expression of interleukin-1β (IL-1β) in rat oligodendrocytes. BIOLOGICAL CHEMISTRY, 380(2), 259-264 [10.1515/BC.1999.034].

Constitutive expression of interleukin-1β (IL-1β) in rat oligodendrocytes

Brogi, A.;Strazza, M.;Luddi, A.;
1999-01-01

Abstract

The RT-PCR analysis of RNA from progenitor and differentiated primary rat oligodendrocytes, and from the oligodendrocyte CG-4 cell line, shows the presence of the IL-1β mRNA, the type I IL-1 receptor and the IL-1 receptor accessory protein in these cells. In situ hybridization of a rat IL-1β probe to primary progenitor and differentiated rat oligodendrocytes results in a positive signal. The double hybridization of the IL-1β probe, together with an oligodendrocyte-specific differentiation marker, to sections of postnatal rat brain at different stages of differentiation is also positive. The double immune-labelling technique utilized indicates coincidence of the signals on the brain slices. The results show that IL-1β mRNA is constitutively expressed in rat brain oligodendrocytes from 1 day after birth onward. In agreement with this observation, CG-4 cells, primary progenitor and differentiated rat oligodendrocytes are positively stained by antibodies against IL-1β. Postnatal brain slices from 1 and 4 day old and adult rats, labelled with a double immunofluorescence technique, are also stained by antibodies against IL-1β. This signal coincides with that of antibodies against oligodendrocyte-specific surface markers. We conclude that IL-1β is constitutively expressed in rat brain progenitor and differentiated oligodendrocytes.
Blasi, F., Riccio, M., Brogi, A., Strazza, M., Taddei, M.L., Romagnoli, S., et al. (1999). Constitutive expression of interleukin-1β (IL-1β) in rat oligodendrocytes. BIOLOGICAL CHEMISTRY, 380(2), 259-264 [10.1515/BC.1999.034].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/1121908
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