Colistin and transition metal ions are commonly used as feed additives for livestock animals. This work presents the results of an analysis of combined potentiometric and spectroscopic (UV-vis, EPR, CD, NMR) data which lead to conclude that colistin is able to effectively chelate copper(II) ions. In cell-free system the oxidative activity of the complex manifests itself in the plasmid DNA destruction with simultaneous generation of reactive (OH)-O-center dot species, when accompanied by hydrogen peroxide or ascorbic acid. The degradation of RNA occurs most likely via a hydrolytic mechanism not only for complexed compound but also colistin alone. Therefore, huge amounts of the used antibiotic for nontherapeutic purposes might have a potential influence on livestock health. (C) 2015 Elsevier Inc. All rights reserved.
Stokowa-Soltys, K., Kasprowicz, A., Wrzesinski, J., Ciesiolka, J., Gaggelli, N., Gaggelli, E., et al. (2015). Impact of Cu2+ ions on the structure of colistin and cell-free system nucleic acid degradation. JOURNAL OF INORGANIC BIOCHEMISTRY, 151, 67-74 [10.1016/j.jinorgbio.2015.05.011].
Impact of Cu2+ ions on the structure of colistin and cell-free system nucleic acid degradation
Gaggelli N.Writing – Original Draft Preparation
;Gaggelli E.Writing – Review & Editing
;Valensin G.Writing – Review & Editing
;
2015-01-01
Abstract
Colistin and transition metal ions are commonly used as feed additives for livestock animals. This work presents the results of an analysis of combined potentiometric and spectroscopic (UV-vis, EPR, CD, NMR) data which lead to conclude that colistin is able to effectively chelate copper(II) ions. In cell-free system the oxidative activity of the complex manifests itself in the plasmid DNA destruction with simultaneous generation of reactive (OH)-O-center dot species, when accompanied by hydrogen peroxide or ascorbic acid. The degradation of RNA occurs most likely via a hydrolytic mechanism not only for complexed compound but also colistin alone. Therefore, huge amounts of the used antibiotic for nontherapeutic purposes might have a potential influence on livestock health. (C) 2015 Elsevier Inc. All rights reserved.File | Dimensione | Formato | |
---|---|---|---|
1-s2.0-S0162013415300039-main.pdf
non disponibili
Tipologia:
PDF editoriale
Licenza:
NON PUBBLICO - Accesso privato/ristretto
Dimensione
1.05 MB
Formato
Adobe PDF
|
1.05 MB | Adobe PDF | Visualizza/Apri Richiedi una copia |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
https://hdl.handle.net/11365/1083094