Polycyclic aromatic hydrocarbons (PAHs), such as naphthalene, are potential health risks due to their carcinogenic and mutagenic effects. Bacteria from the genus Rhodococcus are able to metabolise a wide variety of pollutants such as alkanes, aromatic compounds and halogenated hydrocarbons. A naphthalene dioxygenase from Rhodococcus sp. strain NCIMB12038 has been characterised for the first time, using electron paramagnetic resonance (EPR) spectroscopy and UV-Vis spectrophotometry. In the native state, the EPR spectrum of naphthalene 1,2-dioxygenase (NDO) is formed of the mononuclear high spin Fe(III) state contribution and the oxidised Rieske cluster is not visible as EPR-silent. In the presence of the reducing agent dithionite a signal derived from the reduction of the [2Fe-2S] unit is visible. The oxidation of the reduced NDO in the presence of O2-saturated naphthalene increased the intensity of the mononuclear contribution. A study of the “peroxide shunt”, an alternative mechanism for the oxidation of substrate in the presence of H2O2, showed catalysis via the oxidation of mononuclear centre while the Rieske-type cluster is not involved in the process. Therefore, the ability of these enzymes to degrade recalcitrant aromatic compounds makes them suitable for bioremediative applications and synthetic purposes.
|Titolo:||Spectroscopic characterisation of the naphthalene dioxygenase from Rhodococcus sp. StrainNCIMB12038|
BARATTO, MARIA CAMILLA (Corresponding)
|Citazione:||Baratto, M.C., Lipscomb, D.A., Larkin, M.J., Basosi, R., Allen, C.C.R., & Pogni, R. (2019). Spectroscopic characterisation of the naphthalene dioxygenase from Rhodococcus sp. StrainNCIMB12038. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 20(14), 1-9.|
|Appare nelle tipologie:||1.1 Articolo in rivista|