The roles of the bcl-2 and p53 proteins in Hodgkin's disease (HD) are poorly understood. We therefore compared their detected presence in Hodgkin and Reed-Sternberg/large atypical (H-RS/LA) cells immunohistochemically with the percentages of these cells double-labeled for CD30 and DNA strand breaks (DNA fragmentation index, DFI); mitotic indices (MI); and the EBV infection status. We found a highly significant inverse correlation between the fractions per case of H-RS/LA cells expressing bcl-2/p53 proteins and the DFI of CD30(+) elements. No marked effect of these two oncoproteins on MI was noticed, although these parameters and DFI of CD30(+) cells were linearly related. EBV infection of H-RS/LA cells exerted only a limited effect on the parameters tested. The results of this study suggest that overexpressed bcl-2 and, to some extent, p53 proteins in H-RS/LA cells of HD primarily counteract deletion of these cells.
Leoncini, L., Spina, D., Close, P., Minacci, C., Megha, T., De Luca, F., et al. (1997). Mitotic activity and nuclear DNA damage of large cells in Hodgkin's disease: Comparison with the expression of p53 and bcl-2 proteins and the presence of Epstein-Barr virus. LEUKEMIA & LYMPHOMA, 25(1-2), 153-161 [10.3109/10428199709042505].
Mitotic activity and nuclear DNA damage of large cells in Hodgkin's disease: Comparison with the expression of p53 and bcl-2 proteins and the presence of Epstein-Barr virus
Leoncini L.;Spina D.;Minacci C.;Megha T.;Tosi P.;
1997-01-01
Abstract
The roles of the bcl-2 and p53 proteins in Hodgkin's disease (HD) are poorly understood. We therefore compared their detected presence in Hodgkin and Reed-Sternberg/large atypical (H-RS/LA) cells immunohistochemically with the percentages of these cells double-labeled for CD30 and DNA strand breaks (DNA fragmentation index, DFI); mitotic indices (MI); and the EBV infection status. We found a highly significant inverse correlation between the fractions per case of H-RS/LA cells expressing bcl-2/p53 proteins and the DFI of CD30(+) elements. No marked effect of these two oncoproteins on MI was noticed, although these parameters and DFI of CD30(+) cells were linearly related. EBV infection of H-RS/LA cells exerted only a limited effect on the parameters tested. The results of this study suggest that overexpressed bcl-2 and, to some extent, p53 proteins in H-RS/LA cells of HD primarily counteract deletion of these cells.
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https://hdl.handle.net/11365/1076853