We re-appraised the cell renewal pattern in Hodgkin's disease (HD), considering that most, though not all, Hodgkin/Reed-Sternberg (H-RS) cells exhibit abortive mitoses and that a substantial fraction of these exhibits DNA damage suggestive of imminent or actual cell death. Using combined immunohistochemistry and in situ end-labeling to detect strand breaks, the percentage per case of CD30+ (mainly H-RS) cells with DNA fragmentation (DNA fragmentation index [DFI]) was estimated. For each case, we registered the mitotic index (MI) of CD30+ cells and the percentage of Ki-67+ atypical large cells. To quantify the sum of our parameters for mitosis, whether successful or not, and DNA damage, we introduced the kinetic event index (KEI = MI + DFI). Only DFI and KEI distinguished significantly between mixed cellularity and nodular sclerosis HD. The values for MI and DFI, and therefore for KEI, of CD30+ and CD30- small lymphoid cells were proportional. The percentages of Ki-67+ large atypical cells (median 50%) did not correlate significantly with either MIs or DFIs of CD30+ cells. Cluster analysis revealed the existence, independent of histological subtype, of 2 large groups of HD with different KEIs. Our findings suggest that cell deletion plays an important role in HD. Further, it appears that proliferation-associated antigens in H-RS cells do not reflect successful cell production in this disorder.

Spina, D., Leoncini, L., Close, P., Megha, T., Pacenti, L., Tosi, P., et al. (1996). Growth vs. DNA strand breaks in Hodgkin's disease: Impaired proliferative ability of Hodgkin and Reed-Sternberg cells. INTERNATIONAL JOURNAL OF CANCER, 66(2), 179-183 [10.1002/(SICI)1097-0215(19960410)66:2<179::AID-IJC7>3.0.CO;2-#].

Growth vs. DNA strand breaks in Hodgkin's disease: Impaired proliferative ability of Hodgkin and Reed-Sternberg cells

Spina D.;Leoncini L.;Megha T.;Pacenti L.;Tosi P.;
1996-01-01

Abstract

We re-appraised the cell renewal pattern in Hodgkin's disease (HD), considering that most, though not all, Hodgkin/Reed-Sternberg (H-RS) cells exhibit abortive mitoses and that a substantial fraction of these exhibits DNA damage suggestive of imminent or actual cell death. Using combined immunohistochemistry and in situ end-labeling to detect strand breaks, the percentage per case of CD30+ (mainly H-RS) cells with DNA fragmentation (DNA fragmentation index [DFI]) was estimated. For each case, we registered the mitotic index (MI) of CD30+ cells and the percentage of Ki-67+ atypical large cells. To quantify the sum of our parameters for mitosis, whether successful or not, and DNA damage, we introduced the kinetic event index (KEI = MI + DFI). Only DFI and KEI distinguished significantly between mixed cellularity and nodular sclerosis HD. The values for MI and DFI, and therefore for KEI, of CD30+ and CD30- small lymphoid cells were proportional. The percentages of Ki-67+ large atypical cells (median 50%) did not correlate significantly with either MIs or DFIs of CD30+ cells. Cluster analysis revealed the existence, independent of histological subtype, of 2 large groups of HD with different KEIs. Our findings suggest that cell deletion plays an important role in HD. Further, it appears that proliferation-associated antigens in H-RS cells do not reflect successful cell production in this disorder.
Spina, D., Leoncini, L., Close, P., Megha, T., Pacenti, L., Tosi, P., et al. (1996). Growth vs. DNA strand breaks in Hodgkin's disease: Impaired proliferative ability of Hodgkin and Reed-Sternberg cells. INTERNATIONAL JOURNAL OF CANCER, 66(2), 179-183 [10.1002/(SICI)1097-0215(19960410)66:2<179::AID-IJC7>3.0.CO;2-#].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/1076833