This study was undertaken to better comprehend the reasons for the scarcity of Hodgkin and Reed-Sternberg (H-RS) cells in Hodgkin's disease (HD) despite their expression of ''proliferation-associated antigens''. To this end, we assessed the relative frequency of mitotic phases and nuclear damage (detected by in situ end-labeling of DNA strand breaks) in CD30(+) large cells of nodular sclerosis and mixed cellularity HD. Our results show that a) most CD3(+) cells in HD exhibit abortive mitoses, with a highly significant arrest at the metaphase-ana/telophase transition, and b) many of these elements, i.e. mainly H-RS cells, show fragmentation of nuclear DNA, suggesting imminent or actual death. Percentages of CD30(+) cells that entered mitosis and those with DNA strand breaks were of a similar order of magnitude and correlated significantly in a linear fashion. These findings are consistent with the concept that cell deletion is the major cause of the paucity of H-RS cells in HD.

Leoncini, L., Spina, D., Close, P., Megha, T., Pacenti, L., Tosi, P., et al. (1996). Abortive mitoses and nuclear DNA fragmentation in CD30+ large cells of Hodgkin's disease. LEUKEMIA & LYMPHOMA, 22(1-2), 119-124 [10.3109/10428199609051738].

Abortive mitoses and nuclear DNA fragmentation in CD30+ large cells of Hodgkin's disease

Leoncini L.;Spina D.;Megha T.;Pacenti L.;Tosi P.;
1996-01-01

Abstract

This study was undertaken to better comprehend the reasons for the scarcity of Hodgkin and Reed-Sternberg (H-RS) cells in Hodgkin's disease (HD) despite their expression of ''proliferation-associated antigens''. To this end, we assessed the relative frequency of mitotic phases and nuclear damage (detected by in situ end-labeling of DNA strand breaks) in CD30(+) large cells of nodular sclerosis and mixed cellularity HD. Our results show that a) most CD3(+) cells in HD exhibit abortive mitoses, with a highly significant arrest at the metaphase-ana/telophase transition, and b) many of these elements, i.e. mainly H-RS cells, show fragmentation of nuclear DNA, suggesting imminent or actual death. Percentages of CD30(+) cells that entered mitosis and those with DNA strand breaks were of a similar order of magnitude and correlated significantly in a linear fashion. These findings are consistent with the concept that cell deletion is the major cause of the paucity of H-RS cells in HD.
Leoncini, L., Spina, D., Close, P., Megha, T., Pacenti, L., Tosi, P., et al. (1996). Abortive mitoses and nuclear DNA fragmentation in CD30+ large cells of Hodgkin's disease. LEUKEMIA & LYMPHOMA, 22(1-2), 119-124 [10.3109/10428199609051738].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/1076790