Lomefloxacin (LF) is interesting as a model molecule from a safety point of view because of its high potential for serious adverse drug effects (i.e. phototoxic reactions). In this study, MCM-41 mesoporous silica nanoparticles (MCM-41) were loaded with lomefloxacin, aiming to overcome the drug's intrinsic cytotoxicity. The good biocompatibility of the empty drug carrier (0.1-1.0 mg/ml) was established by the absence of red blood cell lysis (hemolysis assay). The cytotoxicity of empty MCM-41 and lomefloxacin-loaded MCM-41 (LF-MCM-41) was evaluated by using a battery of in vitro cytotoxicity assays: Alamar blue, lactate dehydrogenase release and reactive oxygen species formation by dichlorofluorescein assay. Three cell cultures models: hepatoma HepG2, fibroblasts L929 and endothelial EA.hy926 cells were used to compare the cytotoxicity and reactive oxygen species formation by free drug, empty MCM-41, and LF-MCM-41. The findings from the study indicated that empty MCM-41 (0.1-1.0 mg/ml) showed a low cytotoxic potential in HepG2, followed by L929 and EA.hy926 cells. Lomefloxacin loading in MCM-41 mesoporous silica nanocarrier reduced the cytotoxicity of the free lomefloxacin, especially in the high concentration (1.0 mg/ml MCM-41, containing 120 µg/ml LF). L929 and EA.hy926 cells were more sensitive to the protective effects of LF-MCM-41, compared to HepG2 cells. The results indicate that an improvement in lomefloxacin safety might be expected after incorporation in an appropriate drug delivery system.
Tzankova, V., Aluani, D., Yordanov, Y., Valoti, M., Frosini, M., Spassova, I., et al. (2019). In vitro toxicity evaluation of lomefloxacin-loaded MCM-41 mesoporous silica nanoparticles. DRUG AND CHEMICAL TOXICOLOGY, 1-12.
|Titolo:||In vitro toxicity evaluation of lomefloxacin-loaded MCM-41 mesoporous silica nanoparticles|
|Citazione:||Tzankova, V., Aluani, D., Yordanov, Y., Valoti, M., Frosini, M., Spassova, I., et al. (2019). In vitro toxicity evaluation of lomefloxacin-loaded MCM-41 mesoporous silica nanoparticles. DRUG AND CHEMICAL TOXICOLOGY, 1-12.|
|Appare nelle tipologie:||1.1 Articolo in rivista|