The Single Radial Haemolysis (SRH) assay is a serological method widely used for measuring antibodies against influenza viruses. Despite the broad application and recommendation by licensing authorities, the SRH assay has not been standardized.The aim of this study is to demonstrate how the SRH assay satisfies validation parameters of regulatory agencies in terms of specificity, precision, repeatability, intermediate precision, linearity, accuracy and robustness. This study shows that the SRH is a rapid, simple, reliable and reproducible assay, which requires only small volumes of serum samples and can be easily standardized.

Trombetta, C.M., Perini, D., Vitale, L., Cox, R.J., Stanzani, V., Piccirella, S., et al. (2015). Validation of Single Radial Haemolysis assay: A reliable method to measure antibodies against influenza viruses. JOURNAL OF IMMUNOLOGICAL METHODS, 422, 95-101 [10.1016/j.jim.2015.04.009].

Validation of Single Radial Haemolysis assay: A reliable method to measure antibodies against influenza viruses

Trombetta, Claudia Maria;Montomoli, Emanuele
2015

Abstract

The Single Radial Haemolysis (SRH) assay is a serological method widely used for measuring antibodies against influenza viruses. Despite the broad application and recommendation by licensing authorities, the SRH assay has not been standardized.The aim of this study is to demonstrate how the SRH assay satisfies validation parameters of regulatory agencies in terms of specificity, precision, repeatability, intermediate precision, linearity, accuracy and robustness. This study shows that the SRH is a rapid, simple, reliable and reproducible assay, which requires only small volumes of serum samples and can be easily standardized.
Trombetta, C.M., Perini, D., Vitale, L., Cox, R.J., Stanzani, V., Piccirella, S., et al. (2015). Validation of Single Radial Haemolysis assay: A reliable method to measure antibodies against influenza viruses. JOURNAL OF IMMUNOLOGICAL METHODS, 422, 95-101 [10.1016/j.jim.2015.04.009].
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11365/1059328