TGF-β concentrations and activity are down-regulated in the aqueous humor of patients with neovascular age-related macular degeneration

Tosi, Gian Marco; Neri, Giovanni; Caldi, Elena; Fusco, Fiorella; Bacci, Tommaso; Tarantello, Antonio; Nuti, Elisabetta; Marigliani, Davide; Baiocchi, Stefano; Traversi, Claudio; Barbarino, Marcella; Eandi, Chiara M.; Parolini, Barbara; Mundo, Lucia; Santucci, Annalisa; Orlandini, Maurizio; Galvagni, Federico

doi:10.1038/s41598-018-26442-0

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Controversy still exists regarding the role of the TGF-β in neovascular age-related macular degeneration (nAMD), a major cause of severe visual loss in the elderly in developed countries. Here, we measured the concentrations of active TGF-β1, TGF-β2, and TGF-β3 by ELISA in the aqueous humor of 20 patients affected by nAMD, who received 3 consecutive monthly intravitreal injections of anti-VEGF-A antibody. Samples were collected at baseline (before the first injection), month 1 (before the second injection), and month 2 (before the third injection). The same samples were used in a luciferase-based reporter assay to test the TGF-β pathway activation. Active TGF-β1 concentrations in the aqueous humor were below the minimum detectable dose. Active TGF-β2 concentrations were significantly lower at baseline and at month 1, compared to controls. No significant differences in active TGF-β3 concentration were found among the sample groups. Moreover, TGF-β pathway activation was significantly lower at baseline compared to controls. Our data corroborate an anti-angiogenic role for TGF-β2 in nAMD. This should be considered from the perspective of a therapy using TGF-β inhibitors.

Titolo:	TGF-β concentrations and activity are down-regulated in the aqueous humor of patients with neovascular age-related macular degeneration
Autori:	TOSI, GIAN MARCO NERI, GIOVANNI Caldi, Elena Fusco, Fiorella BACCI, TOMMASO TARANTELLO, ANTONIO NUTI, ELISABETTA Marigliani, Davide BAIOCCHI, STEFANO TRAVERSI, CLAUDIO BARBARINO, MARCELLA Eandi, Chiara M. Parolini, Barbara MUNDO, LUCIA SANTUCCI, ANNALISA ORLANDINI, MAURIZIO (Corresponding) GALVAGNI, FEDERICO (Corresponding) mostra contributor esterni nascondi contributor esterni
Rivista:	SCIENTIFIC REPORTS
Citazione:	Tosi, G.M., Neri, G., Caldi, E., Fusco, F., Bacci, T., Tarantello, A., et al. (2018). TGF-β concentrations and activity are down-regulated in the aqueous humor of patients with neovascular age-related macular degeneration. SCIENTIFIC REPORTS, 8(1), 1-9.
Anno:	2018
Appare nelle tipologie:	1.1 Articolo in rivista

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