To investigate the effects of two non-protein amino acids (NPAAs), β-alanine and γ-amino butyric acid (GABA), on Osmia bicornis survival and locomotion, two groups of ten caged bees have been fed with a sugar syrup enriched with β-alanine and GABA, respectively. The further control group was fed with the sugar syrup. Five behavioural categories have been chosen in accordance with the principle of parsimony and with their intrinsic unitary consistency from onset to the end. Two states (staying under the paper and staying in the test tubes) and three events (walking on the net, feeding on the flower and flying) have been recorded by scan sampling. Furthermore, we analysed the amino acids content of the haemolymph that was sampled from additional forty-five bees kept under the same diet condition (15 per diet type). Bees fed with ß-alanine had a significant shorter survival than those fed with control and GABA diets. The GABA diet induced a higher locomotion than the β-alanine. The former non-protein amino acid was detected in the haemolymph of only bees fed with GABA. Results suggest that insects consuming NPAAs-reach diets can absorb and transfer these substances into the haemolymph and those NPAAs affect both survival and locomotion. Ecological consequences are discussed in the frame of plant reproductive biology.
|Titolo:||Effects of nonprotein amino acids on survival and locomotion of Osmia bicornis|
NEPI, MASSIMO (Corresponding)
|Appare nelle tipologie:||1.1 Articolo in rivista|