RAPDs-fingerprinting was used to differentiate 9 olive genotypes from central Italy (Tuscany and Liguria). OPA-09 and OPA-13 both generated a high degree of polymorphism in all samples. DNA markers were evidentiated by agarose-gel electrophoresis and ethidiumbromide staining. In parallel DNA was extracted from monovarietal oil sediments, subjected to RAPD-PCR amplification and subsequently compared to the leaf fingerprint belonging to the same cultivar. DNA markers profiles of amplified samples of oil and leaf extracts, obtained with primer OPA-90 from genotype Grossolana showed a high degree of similarity.
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|Titolo:||Preliminary communication about the identification of DNA in leaves and in olive oil of Olea europaea|
|Appare nelle tipologie:||1.1 Articolo in rivista|