RAPDs-fingerprinting was used to differentiate 9 olive genotypes from central Italy (Tuscany and Liguria). OPA-09 and OPA-13 both generated a high degree of polymorphism in all samples. DNA markers were evidentiated by agarose-gel electrophoresis and ethidiumbromide staining. In parallel DNA was extracted from monovarietal oil sediments, subjected to RAPD-PCR amplification and subsequently compared to the leaf fingerprint belonging to the same cultivar. DNA markers profiles of amplified samples of oil and leaf extracts, obtained with primer OPA-90 from genotype Grossolana showed a high degree of similarity.
Cresti, M., Linskens, H.F., Mulcahy, D.L., Bush, S., Di Stilio, V., Xu, M.Y., et al. (1996). Preliminary communication about the identification of DNA in leaves and in olive oil of Olea europaea. ADVANCES IN HORTICULTURAL SCIENCE(2), 105-107.
Preliminary communication about the identification of DNA in leaves and in olive oil of Olea europaea
Cresti, M.;Vignani, R.;
1996-01-01
Abstract
RAPDs-fingerprinting was used to differentiate 9 olive genotypes from central Italy (Tuscany and Liguria). OPA-09 and OPA-13 both generated a high degree of polymorphism in all samples. DNA markers were evidentiated by agarose-gel electrophoresis and ethidiumbromide staining. In parallel DNA was extracted from monovarietal oil sediments, subjected to RAPD-PCR amplification and subsequently compared to the leaf fingerprint belonging to the same cultivar. DNA markers profiles of amplified samples of oil and leaf extracts, obtained with primer OPA-90 from genotype Grossolana showed a high degree of similarity.
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https://hdl.handle.net/11365/1030170