RAPDs-fingerprinting was used to differentiate 9 olive genotypes from central Italy (Tuscany and Liguria). OPA-09 and OPA-13 both generated a high degree of polymorphism in all samples. DNA markers were evidentiated by agarose-gel electrophoresis and ethidiumbromide staining. In parallel DNA was extracted from monovarietal oil sediments, subjected to RAPD-PCR amplification and subsequently compared to the leaf fingerprint belonging to the same cultivar. DNA markers profiles of amplified samples of oil and leaf extracts, obtained with primer OPA-90 from genotype Grossolana showed a high degree of similarity.
|Titolo:||Preliminary communication about the identification of DNA in leaves and in olive oil of Olea europaea|
|Citazione:||Cresti, M., Linskens, H.F., Mulcahy, D.L., Bush, S., Di Stilio, V., Xu, M.Y., et al. (1996). Preliminary communication about the identification of DNA in leaves and in olive oil of Olea europaea. ADVANCES IN HORTICULTURAL SCIENCE(2), 105-107.|
|Appare nelle tipologie:||1.1 Articolo in rivista|