Microsatellite (MS) VVMD21 (BOWERS et al. 1999) was taken as a model to explore the molecular basis of polymorphism in a panel of 6 grapevine accessions (Vitis vinifera L.), consisting of Sangiovese and Cabernet Sauvignon and 4 F1 plants derived from crossing both varieties. The 12 alleles of both parents and the progeny were cloned and sequenced. The microsatellite repeat (AG)n>6 was found in each sequence, together with a poly-T rich region that showed irregularity. Furthermore, single nucleotide deletion or exchange (point mutations) were found in the microsatellite flanking regions.
Masi, E., Vignani, R., Sensi, E., Scali, M., Cresti, M., Boselli, M. (2004). Sequence analysis of a microsatellite and its flanking regions in intraspecific hybrids of grapevine (Vitis vinifera L.). VITIS, 43(2), 69-73.
Sequence analysis of a microsatellite and its flanking regions in intraspecific hybrids of grapevine (Vitis vinifera L.)
Vignani, R.;Scali, M.;Cresti, M.;
2004-01-01
Abstract
Microsatellite (MS) VVMD21 (BOWERS et al. 1999) was taken as a model to explore the molecular basis of polymorphism in a panel of 6 grapevine accessions (Vitis vinifera L.), consisting of Sangiovese and Cabernet Sauvignon and 4 F1 plants derived from crossing both varieties. The 12 alleles of both parents and the progeny were cloned and sequenced. The microsatellite repeat (AG)n>6 was found in each sequence, together with a poly-T rich region that showed irregularity. Furthermore, single nucleotide deletion or exchange (point mutations) were found in the microsatellite flanking regions.File | Dimensione | Formato | |
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https://hdl.handle.net/11365/1030121