The identification of proteins involved in pollen germination and tube growth is important for fundamental studies of fertility and reproduction in flowering plants. We used 2-DE and MALDI-TOF-MS to identify differentially expressed proteins in mature (P0) and 1-h germinated (P1) maize pollen. Among about 470 proteins separated in 2D gels, the abundances of 26 protein spots changed (up- or down-regulation) between P0 and P1. The 13 up-regulated protein spots were mainly involved in tube wall modification, actin cytoskeleton organization, energy metabolism, signaling, protein folding and degradation. In particular, pectin methylesterase, inorganic pyrophosphatase, glucose-1-phosphate uridylyltransferase and rab GDP dissociation inhibitor Î± are highly up-regulated, suggesting their potential role in pollen tube growth. The down-regulated 13 protein spots mainly include defense-related proteins, pollen allergens and some metabolic enzymes. This study would contribute to the understanding of the changes in protein expression associated with pollen tube development. Â© 2010 Franciszek GÃ³rski Institute of Plant Physiology, Polish Academy of Sciences, KrakÃ³w.
|Titolo:||Proteomic identification of differentially expressed proteins in mature and germinated maize pollen|
|Citazione:||Proteomic identification of differentially expressed proteins in mature and germinated maize pollen / Zhu, Yunhao; Zhao, Pengfei; Wu, Xiaolin; Wang, Wei; Scali, Monica; Cresti, Mauro. - In: ACTA PHYSIOLOGIAE PLANTARUM. - ISSN 0137-5881. - ELETTRONICO. - 33:4(2011), pp. 1467-1474.|
|Appare nelle tipologie:||1.1 Articolo in rivista|
File in questo prodotto: