Glucocorticoids play a primary role in the maturation of fetal organs and may contribute to the onset of labour. Glucocorticoid activity depends on the 11β-hydroxysteroid dehydrogenase family (11β-HSDs), catalysing the interconversion between “active” cortisol into inactive cortisone. No definitive study exists on 11β-HSD expression profile in human decidua and myometrium during pregnancy. We investigated the implications of 11β-HSD1 in the regulation of uterine activity in pregnancy, examining its role on contraction of a myocyte cell line and murine 11β-hsd1 levels in utero. Murine 11β-hsd1 mRNA and protein levels in utero progressively increased until the last day of gestation and significantly decreased at the onset of labour (P < 0.0001) (n = 3 to 5 in the various gestational days analysed). Experiments on human myometrial samples confirm the significant fall in 11β-hsd1 mRNA levels at labour, compared to end pregnancy samples (n = 5 to 8). In vitro experiments showed that human myometrial contraction is inhibited by using a non-selective inhibitor of 11β-HSD1. The present study shows the temporal localisation of 11β-HSD1 in uterus, highlighting its importance in the timing of gestation and suggesting its contribution in the myometrium contraction.
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|Titolo:||11β-hydroxysteroid dehydrogenase type 1 and pregnancyRole in the timing of labour onset and in myometrial contraction|
|Citazione:||Damiani, F., Makieva, S., Rinaldi, S.F., Hua, L., Marcolongo, P., Petraglia, F., et al. (2017). 11β-hydroxysteroid dehydrogenase type 1 and pregnancyRole in the timing of labour onset and in myometrial contraction. MOLECULAR AND CELLULAR ENDOCRINOLOGY, 447, 79-86.|
|Appare nelle tipologie:||1.1 Articolo in rivista|
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