Detection of allergen-IgE interaction in allergic children through impedance measurements

The aim of this work is to provide an alternative and more efficient screening test in order to monitor the interaction of the IgE protein once in contact with the allergen in an allergic subject. The idea relies on the fact that the interaction between the IgE and the corresponding allergen happens through a bonding mechanism. According to this interaction it is possible that some of ionic bond or electrostatic interaction will happen between the IgE and the allergen structure changing the ionic content of the medium. This latter can be monitored through impedenzimetric measurements. Therefore in this paper we propose a novel system for the detection of allergies based on impedenzimetry This type of measurement instrument has never been presented in the literature for this purpose. Moreover a measurement protocol has been defined to make the measurements reproducible and repeatable over time. The following description addresses both the instrument and the measurement protocol identified for this purpose, defining univocally, in this way, the overall measurement technique.