Benzene, a chemical widely used in industry, is capable of causing aplastic anemia and acute myelogenous leukemia in humans and multiple forms of cancer in rodents. Hepatic cytochrome P450 2E1 catalyzes the formation of phenolic metabolites of benzene. Mielopeoxidase (MPO), present in bone marrow, may further convert the phenolic metabolites to free radicals, which are responsible for in situ bioactivation of benezene. The aim of this work was to study the effects of low and high doses of benzene (5 and 100 mg/Kg) in subchronically treated mice. Treatment with 100 mg/Kg led to a significative increase in MPO and in NAD(P)H:quinone-oxidoreductase (NADHQ). Furthermore "comet assay" in myelocytes revealed DNA damage for both types of benzene treatment.
Dragoni, S., Franceschetti, P., Doria, D., Valoti, M., Fracasso, M.E. (2004). Effects on C57/BL mice of subchronic treatment with benzene [Effetto del trattamento subcronico con benzene in topi C57/Bl]. In Giornale Italiano di Medicina del Lavoro ed Ergonomia (pp.55-56).
Effects on C57/BL mice of subchronic treatment with benzene [Effetto del trattamento subcronico con benzene in topi C57/Bl]
DRAGONI S.;VALOTI M.;
2004-01-01
Abstract
Benzene, a chemical widely used in industry, is capable of causing aplastic anemia and acute myelogenous leukemia in humans and multiple forms of cancer in rodents. Hepatic cytochrome P450 2E1 catalyzes the formation of phenolic metabolites of benzene. Mielopeoxidase (MPO), present in bone marrow, may further convert the phenolic metabolites to free radicals, which are responsible for in situ bioactivation of benezene. The aim of this work was to study the effects of low and high doses of benzene (5 and 100 mg/Kg) in subchronically treated mice. Treatment with 100 mg/Kg led to a significative increase in MPO and in NAD(P)H:quinone-oxidoreductase (NADHQ). Furthermore "comet assay" in myelocytes revealed DNA damage for both types of benzene treatment.
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https://hdl.handle.net/11365/5760
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