Comparison of different solutions for final rinse optimization, Hypoclean

Aim. The purpose of this study was to assess the penetration of a new modified sodium hypochlorite solution labelled with 0.2% alizarin red into dentinal tubules when used in root canals with PUI activation. Methods. 32 extracted human single-rooted teeth were selected for the study. Crowns were at 2 mm above the CEJ. 30.06 was the last file used at the WL. The teeth were randomly divided into four groups (N=8). A final rinse of each canal was performed with different agitation procedure: control, NaOCl+PUI, Hypoclean, Hypoclean+PUI. Specimen was cut at 1, 3, and 5 mm from the apex and were examined with a fluorescence light microscope at 100X. Images were evaluated following a set of scores used to assess the penetration of solution into the dentinal tubules. Statistical analysis was performed by using Kruskal-Wallis analysis. Results. Differences were found among groups in relation to the irrigation regimen used. Groups were ranked in the following order: control<Hypoclean<NaOCl+PUI= Hypoclean+PUI Group. At 1 mm from the root apex, the Hypoclean+PUI group exhibited the highest score. Discussion: In the present study Hypoclean was compared to 5.25% NaOCl. The two irrigant were tested without activation into root canal and in association with PUI. Hypoclean showed a better result when was used without activation, but no differences were found when was used in combination with PUI.