Cytokines are regulatory proteins well known for their pleiotropic actions on a variety of cells. It is well known that local hypoxia is a very common condition and it may control the release of cytokines, physiologically relevant in controlling cell proliferation. For these reasons, we decided to study the production of cytokines by resting and phytohaemagglutinin (PHA)-activated peripheral blood mononuclear cells (PBMC), either at normal (20% O22 pO2 140 mmHg) or lower oxygen tension (2% Og pO2 14 mrnHg).Hypoxia significantly modulates the production of cytokines in human PBMC: Interleukin-2 (IL-2), Interleukin-4 (IL-4) and gamma-interferon (IFN) production increased 110, 70 and 50% of controls, respectively, in PHA-stimulated PBMC . Moreover, in hypoxia, IL-6 production was significantly enhanced in both resting and PHA-stimulated PBMC by 36 and 37%, respectively. On the other hand, in hypoxia IL-10 production decreased in both resting and stimulated PBMC, being 80 and 67% of controls, respectively. PBMC proliferation was not significantly affected by hypoxia, even though PBMC susceptibility to PHA was about 80% of controls (p<0.05), after 40 hr of treatment. By evaluating these data, it appears that hypoxia modifies the production of cytokines by PBMC. These results have both theoretical and practical interest because local hypoxia is very common in several conditions, as inflammation, local ischemia and is also a host nonspecific defense against infection. Further, they suggest a differential pattern of cytokine production "in vivo" in hypoxic tissues.

Carraro, F., Bocci, V., Fanetti, G., Naldini, A. (1997). Hypoxia modulates the release of cytokines by human peripheral blood mononuclear cells. THE FASEB JOURNAL, 11(3), A428-A428.

Hypoxia modulates the release of cytokines by human peripheral blood mononuclear cells

CARRARO, F.;BOCCI, V.;NALDINI, A.
1997-01-01

Abstract

Cytokines are regulatory proteins well known for their pleiotropic actions on a variety of cells. It is well known that local hypoxia is a very common condition and it may control the release of cytokines, physiologically relevant in controlling cell proliferation. For these reasons, we decided to study the production of cytokines by resting and phytohaemagglutinin (PHA)-activated peripheral blood mononuclear cells (PBMC), either at normal (20% O22 pO2 140 mmHg) or lower oxygen tension (2% Og pO2 14 mrnHg).Hypoxia significantly modulates the production of cytokines in human PBMC: Interleukin-2 (IL-2), Interleukin-4 (IL-4) and gamma-interferon (IFN) production increased 110, 70 and 50% of controls, respectively, in PHA-stimulated PBMC . Moreover, in hypoxia, IL-6 production was significantly enhanced in both resting and PHA-stimulated PBMC by 36 and 37%, respectively. On the other hand, in hypoxia IL-10 production decreased in both resting and stimulated PBMC, being 80 and 67% of controls, respectively. PBMC proliferation was not significantly affected by hypoxia, even though PBMC susceptibility to PHA was about 80% of controls (p<0.05), after 40 hr of treatment. By evaluating these data, it appears that hypoxia modifies the production of cytokines by PBMC. These results have both theoretical and practical interest because local hypoxia is very common in several conditions, as inflammation, local ischemia and is also a host nonspecific defense against infection. Further, they suggest a differential pattern of cytokine production "in vivo" in hypoxic tissues.
1997
Carraro, F., Bocci, V., Fanetti, G., Naldini, A. (1997). Hypoxia modulates the release of cytokines by human peripheral blood mononuclear cells. THE FASEB JOURNAL, 11(3), A428-A428.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/27811
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