Besides being markers of oxidative stress, F2-isoprostanes (IsoPs) proved to be mediators of biological effects through for thromboxane A2 receptors (TxA2r). We showed that IsoPs, generated during CCl4-induced hepatic fibrosis, mediate hepatic stellate cell (HSC) proliferation and collagen hyperproduction. Binding studies suggested that the stimulatory effects of 8-epi-PGF2alpha on DNA and collagen synthesis are mediated by a receptor analogous to TxA2r (TP). Moreover, western blotting and immunocytochemistry analysis revealed the expression of TP in HSC both in plasma membranes and within the cells. Studies of the signal transduction pathways set into motion by IsoPs in HSC showed that 8-epi-PGF2alphainduced an increase of Ins(1,4,5)P3 and the activation of ERK1/2, cyclin D1 and other pathways involved in cell proliferation and collagen expression.

Arezzini, B., ., V.D., Gardi, C., Signorini, C., ., M.B., & . Comporti M, . (2008). Oxidative stress and lung fibrosis. In atti del 24° Congresso della Società Italiana di Patologia (pp.5-5).

Oxidative stress and lung fibrosis

GARDI, CONCETTA;SIGNORINI, CINZIA;
2008

Abstract

Besides being markers of oxidative stress, F2-isoprostanes (IsoPs) proved to be mediators of biological effects through for thromboxane A2 receptors (TxA2r). We showed that IsoPs, generated during CCl4-induced hepatic fibrosis, mediate hepatic stellate cell (HSC) proliferation and collagen hyperproduction. Binding studies suggested that the stimulatory effects of 8-epi-PGF2alpha on DNA and collagen synthesis are mediated by a receptor analogous to TxA2r (TP). Moreover, western blotting and immunocytochemistry analysis revealed the expression of TP in HSC both in plasma membranes and within the cells. Studies of the signal transduction pathways set into motion by IsoPs in HSC showed that 8-epi-PGF2alphainduced an increase of Ins(1,4,5)P3 and the activation of ERK1/2, cyclin D1 and other pathways involved in cell proliferation and collagen expression.
Arezzini, B., ., V.D., Gardi, C., Signorini, C., ., M.B., & . Comporti M, . (2008). Oxidative stress and lung fibrosis. In atti del 24° Congresso della Società Italiana di Patologia (pp.5-5).
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11365/26192
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