F2-isoprostanes are markers of oxidative stress and mediators of important biological effects. Previously, we provided evidence that F2-isoprostanes, generated during CCl4-induced hepatic fibrosis, mediate hepatic stellate cell (HSC) proliferation and collagen hyperproduction. We suggested the involvement of a modified form of isoprostane receptor, homologous to the classic TxA2 binding site. The stimulatory effects of 8-epi-PGF2alpha on DNA and collagen synthesis are mediated by TxA2 receptor (TP). Moreover, western blotting and immunocytochemistry analysis revealed the expression of TP on HSC both on plasma membranes and within the cells. Experiments on the signal transduction pathways showed that 8-epi-PGF2alpha increase Ins(1,4,5)P3 and MAPK. Thus, it is likely that the fibrogenic effects induced by 8-epi-PGF2alpha in HSC are mediated by these transduction pathways.

Arezzini, B., Monaco, B., Gardi, C., Vecchio, D., Comporti, M. (2008). F2-Isoprostane receptors and signal transduction on hepatic stellate cells. In atti del 24° Congresso della Società Italiana di Patologia (pp.4-4).

F2-Isoprostane receptors and signal transduction on hepatic stellate cells

Arezzini, B.;Gardi, C.;Vecchio, D.;Comporti, M.
2008-01-01

Abstract

F2-isoprostanes are markers of oxidative stress and mediators of important biological effects. Previously, we provided evidence that F2-isoprostanes, generated during CCl4-induced hepatic fibrosis, mediate hepatic stellate cell (HSC) proliferation and collagen hyperproduction. We suggested the involvement of a modified form of isoprostane receptor, homologous to the classic TxA2 binding site. The stimulatory effects of 8-epi-PGF2alpha on DNA and collagen synthesis are mediated by TxA2 receptor (TP). Moreover, western blotting and immunocytochemistry analysis revealed the expression of TP on HSC both on plasma membranes and within the cells. Experiments on the signal transduction pathways showed that 8-epi-PGF2alpha increase Ins(1,4,5)P3 and MAPK. Thus, it is likely that the fibrogenic effects induced by 8-epi-PGF2alpha in HSC are mediated by these transduction pathways.
Arezzini, B., Monaco, B., Gardi, C., Vecchio, D., Comporti, M. (2008). F2-Isoprostane receptors and signal transduction on hepatic stellate cells. In atti del 24° Congresso della Società Italiana di Patologia (pp.4-4).
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/24708
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