Elevated levels of vascular endothelial growth factor (VEGF) are implicate in progression of diabetic retinopathy. Celecoxib, a COX-2 inhibitor, reduces retinal VEGF expression in a diabetic rat model. The primary goal of this study was to determine in rat the celecoxib disposition in various ocular compartments after intraperitoneal administration. Albino Wistar rats (200 g weight) were used and treated with 3 mg drug. Celecoxib was suspended in 0.5% w/v of carboxy methyl cellulose sodium salt and administered (3mg/rat) to unanaesthetized rat. The animals after pentobarbital (250 mg/kg) were sacrificed at 0.5, 1,2,3,4,8 and 12 hours after celecoxib administration. The plasma was collected and the eyes enucleated immediately and frozen at –80°C. Cornea, lens retina, vitreous and sclera were isolated and drug levels in ocular tissue and plasma were estimated using HPLC method and the concentration-time profiles analysed by compartmental analysis. The plasma and tissue pharmacokinetics parameters of celecoxib were reported.

Pannini, S., Runci, F.M., Martini, L., Urso, R., Giorgi, G. (2005). Comportamental model of celecoxib in rat eye. In Abstract 32° Congresso Nazionale della Società Italiana di Farmacologia (pp.172-172). Societa Italiana di Farmacologia.

Comportamental model of celecoxib in rat eye

PANNINI, SIMONE;RUNCI, FRANCESCO MICHELE;MARTINI, LUCA;GIORGI, GIORGIO
2005-01-01

Abstract

Elevated levels of vascular endothelial growth factor (VEGF) are implicate in progression of diabetic retinopathy. Celecoxib, a COX-2 inhibitor, reduces retinal VEGF expression in a diabetic rat model. The primary goal of this study was to determine in rat the celecoxib disposition in various ocular compartments after intraperitoneal administration. Albino Wistar rats (200 g weight) were used and treated with 3 mg drug. Celecoxib was suspended in 0.5% w/v of carboxy methyl cellulose sodium salt and administered (3mg/rat) to unanaesthetized rat. The animals after pentobarbital (250 mg/kg) were sacrificed at 0.5, 1,2,3,4,8 and 12 hours after celecoxib administration. The plasma was collected and the eyes enucleated immediately and frozen at –80°C. Cornea, lens retina, vitreous and sclera were isolated and drug levels in ocular tissue and plasma were estimated using HPLC method and the concentration-time profiles analysed by compartmental analysis. The plasma and tissue pharmacokinetics parameters of celecoxib were reported.
Pannini, S., Runci, F.M., Martini, L., Urso, R., Giorgi, G. (2005). Comportamental model of celecoxib in rat eye. In Abstract 32° Congresso Nazionale della Società Italiana di Farmacologia (pp.172-172). Societa Italiana di Farmacologia.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/23141
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