Comportamental model of celecoxib in rat eye

Elevated levels of vascular endothelial growth factor (VEGF) are implicate in progression of diabetic retinopathy. Celecoxib, a COX-2 inhibitor, reduces retinal VEGF expression in a diabetic rat model. The primary goal of this study was to determine in rat the celecoxib disposition in various ocular compartments after intraperitoneal administration. Albino Wistar rats (200 g weight) were used and treated with 3 mg drug. Celecoxib was suspended in 0.5% w/v of carboxy methyl cellulose sodium salt and administered (3mg/rat) to unanaesthetized rat. The animals after pentobarbital (250 mg/kg) were sacrificed at 0.5, 1,2,3,4,8 and 12 hours after celecoxib administration. The plasma was collected and the eyes enucleated immediately and frozen at –80°C. Cornea, lens retina, vitreous and sclera were isolated and drug levels in ocular tissue and plasma were estimated using HPLC method and the concentration-time profiles analysed by compartmental analysis. The plasma and tissue pharmacokinetics parameters of celecoxib were reported.