Characterization of Clot Permeability Measurements Varying Sample Realization

The measurement of clot permeability is critical for advancing thrombosis research and improving clinical diagnostics. However, standardized measurement of clots faces several challenges, including the unintentional rupture of clots and the loss of clot samples, leading to unstandardized and inaccurate results. In this study, we propose a novel method for clot realization, utilizing varying concentrations of fibrinogen. We employed a portable standalone measurement setup, previously developed by the authors and further improved in this work. The proposed device measures sample permeability by monitoring the trend of percolated sample fluid through the clot over time. It integrates a closed-loop pressurized fluid control system that dynamically adjusts the fluid pressure above the sample, preventing clot rupture and minimizing sample loss during measurements. Initial results demonstrate the effectiveness of the proposed method and instrument in realizing clots, maintaining their stability, and preserving samples. This approach enhances the accuracy, reliability, and efficiency of clot permeability assessments. The innovative system combines computational modeling with real-time data processing, offering a more stable and reproducible method for assessing clot permeability. This advancement has the potential to set a new standard for thrombosis research and related medical applications, ultimately contributing to better clinical decision-making and patient management. Future research should focus on exploring the system's applicability to more complex biological samples and investigating potential enhancements to increase its sensitivity, accuracy, usability, and non-invasive capabilities.