Analysis of 2ω and 3ω/2 Spectra from Plasmas Produced by Irradiation of Thin Foil Targets

The aim of this paper was to evaluate the 'in vitro' interaction between β-thromboglobulin, one specific platelet release product and polymorphonuclear leukocytes. The effects of β-thromboglobulin were tested on the following features of polymorphonuclear leukocytes activation: opsonized zymosan and phorbol myristate acetate stimulated chemiluminescence, release of membrane bound calcium, NADPH-oxidase activity, and membrane fluidity. Beta-thromboglobulin caused a decrease of the chemiluminescent response of polymorphonuclear leukocytes, using both agonists, with and without preincubation with polymorphonuclear leukocytes. We observed that β-thromboglobulin caused a decrease also in the activity of NADPH-oxidase but not in the release of membrane bound calcium. Moreover β-thromboglobulin caused a decrease of the polymorphonuclear leukocytes membrane fluidity. Our results suggest that the β-thromboglobulin could play a role in the reciprocal interactions between platelets and polymorphonuclear leukocytes.