Due to the constant increase of plastic use and production, microplastics (MPs) have become a contaminant of serious concern for the marine environment. However, detailed information about biological pathways affected by the exposure to different MP polymers is still lacking, in particular at transcriptome level. The present study focused on the identification of the molecular pathways affected by a chronic exposure of zebrafish (Danio rerio) to different concentrations of a combination of two environmentally relevant MPs for 20 days. Adult zebrafish were fed daily with dry fish food (control group, N=12) and food supplemented with a mix of pristine high-density polyethylene and polystyrene microplastics (two experimental groups: 0.1 and 1 mg/L, N=12 each). The microplastics dimension ranged from below 25 µm to 90 µm for both polymers. At the end of the exposure period, the liver was dissected and its whole transcriptome analyzed by next-generation RNA-sequencing technologies on an Illumina platform. In addition, the gastrointestinal tract and the gills were dissected and fixed for histology and immunohistochemistry. The exposure to polyethylene and polystyrene microplastics affected the liver transcriptome in a dose-dependent way, inducing the differential regulation of specific suites of genes. Histological analyses evidenced changes in the inflammatory response occurring at the two mucosal tissues selected for observation. The correlation of histological alterations with differential gene expression will be addressed and discussed. This study provides a comprehensive transcriptomic dataset useful for ecotoxicological studies on other fish species.
|Titolo:||Effects of zebrafish exposure to high-density polyethylene and polystyrene microplastics at molecular and histological levels|
|Citazione:||Limonta, G., Mancia, A., Abelli, L., Fossi, M.C., & Panti, C. (2018). Effects of zebrafish exposure to high-density polyethylene and polystyrene microplastics at molecular and histological levels. In SETAC Europe 28th Annual Meeting - Abstract Book. Responsible and Innovative Research for Environmental Quality (pp.421-422). SETAC Europe.|
|Appare nelle tipologie:||4.2 Abstract in Atti di convegno|
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