Food allergen-IgE impedance measurements evaluation in allergic children

We propose a new strategy for the evaluation of the interaction between IgE proteins and allergens in allergic subjects by impedance measurements. Additionally, reactive oxygen species (ROS) production in leukocyte subpopulations from non-allergic and allergic children was evaluated. On the basis of our results, these new approaches show advantages in terms of diagnostic efficacy with respect to traditional UNICAP and prick skin tests, which are commercially available nowadays. Here we describe in detail the instrument for impedance evaluation and the measurement protocols, defining univocally, in this way, the overall measurement technique.