Colorectal cancer is one of the most common causes of cancer death in the Western world. Most of the colon tumors are sporadic and develop somatically in epithelial cells. Apart from genetic factors, nutritional factors can markedly affect tumor development. In particular, while a high intake of red meat and animal fat is considered key points predisposing to colorectal cancer development, epidemiological studies often observed an inverse correlation between tomato and tomato product (sauce, paste) consumption and colon cancer risk. Tomato antioxidant bioactive molecules such as carotenoids and polyphenols could be responsible, at least in part, for the healthy effect. Here we analyzed the effect of total lipophilic extracts of a Southern Italy tomato variety, Corbarino and its processed form, Corbarino sauce, on two in vitro model of human colorectal adenocarcinoma cell lines, Colo–320 and SW-480, characterized by different aggressiveness. Our results support the hypothesis of a role for this variety of tomato in the inhibition of some features involved in the neoplastic advancement. The treatment with tomato extracts affected cancer cell ability to grow both in adherence and in semisolid medium, reducing also cell migration ability as highlighted after 24 hrs and more relevant results were gained after 72 hrs of incubation. Moreover, the most effective results were obtained with Corbarino sauce extract. No toxic effects were observed on non-tumoral cells, Human Skin Fibroblasts (HSFs). The observed inhibition of cancer cell growth and aggressiveness is associated with a negative regulation of cell cycle progression as pointed out by the increased expression levels of pRb/p105, p107 and pRb2/p130 while p21-Cip1 and p27-Kip1 expression levels decreased. The extent of antineoplastic effects, furthermore, seem to be correlated with the antioxidant activity of the two tomatoes form. Our data indicate that Corbarino tomato and its processed form intake might be further considered as nutritional support not only in cancer prevention, but also for cancer patient diet

Penon, A., Giordano, A. (2017). BIOLOGICAL EFFECTS OF CORBARINO TOMATO LIPOPHILIC EXTRACT AND ITS PROCESSED FORM ON COLORECTAL CANCER CELL LINES AND RELATED MOLECULAR PATHWAY INVOLVED.

BIOLOGICAL EFFECTS OF CORBARINO TOMATO LIPOPHILIC EXTRACT AND ITS PROCESSED FORM ON COLORECTAL CANCER CELL LINES AND RELATED MOLECULAR PATHWAY INVOLVED

PENON, ANTONELLA;GIORDANO, ANTONIO
2017-01-01

Abstract

Colorectal cancer is one of the most common causes of cancer death in the Western world. Most of the colon tumors are sporadic and develop somatically in epithelial cells. Apart from genetic factors, nutritional factors can markedly affect tumor development. In particular, while a high intake of red meat and animal fat is considered key points predisposing to colorectal cancer development, epidemiological studies often observed an inverse correlation between tomato and tomato product (sauce, paste) consumption and colon cancer risk. Tomato antioxidant bioactive molecules such as carotenoids and polyphenols could be responsible, at least in part, for the healthy effect. Here we analyzed the effect of total lipophilic extracts of a Southern Italy tomato variety, Corbarino and its processed form, Corbarino sauce, on two in vitro model of human colorectal adenocarcinoma cell lines, Colo–320 and SW-480, characterized by different aggressiveness. Our results support the hypothesis of a role for this variety of tomato in the inhibition of some features involved in the neoplastic advancement. The treatment with tomato extracts affected cancer cell ability to grow both in adherence and in semisolid medium, reducing also cell migration ability as highlighted after 24 hrs and more relevant results were gained after 72 hrs of incubation. Moreover, the most effective results were obtained with Corbarino sauce extract. No toxic effects were observed on non-tumoral cells, Human Skin Fibroblasts (HSFs). The observed inhibition of cancer cell growth and aggressiveness is associated with a negative regulation of cell cycle progression as pointed out by the increased expression levels of pRb/p105, p107 and pRb2/p130 while p21-Cip1 and p27-Kip1 expression levels decreased. The extent of antineoplastic effects, furthermore, seem to be correlated with the antioxidant activity of the two tomatoes form. Our data indicate that Corbarino tomato and its processed form intake might be further considered as nutritional support not only in cancer prevention, but also for cancer patient diet
2017
Penon, A., Giordano, A. (2017). BIOLOGICAL EFFECTS OF CORBARINO TOMATO LIPOPHILIC EXTRACT AND ITS PROCESSED FORM ON COLORECTAL CANCER CELL LINES AND RELATED MOLECULAR PATHWAY INVOLVED.
Penon, Antonella; Giordano, Antonio
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/1009543
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