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8.1 Tesi Dottorato 1018

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The HNRNPD protein from DNA repair to immune response

Genomic instability is one of the hallmarks of cancer crucial for tumor development and progression. Among the various types of DNA damage, DNA double strand breaks (DSBs) are the most toxic lesions for the genetic material, leading severe consequences on cell viability predisposing to cancer transformation. In order to preserve the genome integrity, two main pathways, homologous recombination (HR) and non-homologous end joining (NHEJ) are activated to repair DSBs. Recent studies highlight a ...

Development and Optimization of a Luminescence-Based Assay to Evaluate Serum Bactericidal Activity Against Neisseria gonorrhoeae

Neisseria gonorrhoeae (Ng) is a human-adapted pathogen and the etiological agent of gonorrhoea, a sexually transmitted infection (STI) that affects millions of people worldwide each year. Over the past two decades, the global incidence of Ng infection has risen, driven by its ability to acquire resistance to available antibiotics. As a result, developing new therapeutic strategies and effective vaccines has become a critical global public health challenge. In this study, we developed a lumin...

Human neural stem cell drug product, culturing and characterization of cell lines for treatment of secondary progressive multiple sclerosis

For several years, human neural stem cells (hNSCs) have been a focus of research, with recent attention to their potential as a cellular treatment for neurodegenerative disorders. The works of Weiss and Reynolds in the 1990s established methods for in vitro cultivation of hNSCs from neural tissue biopsies taken from the subventricular zone (SVZ). Subsequent characterization and development of appropriate potency and safety tests have paved the way for clinical trials exploring the use of thes...

Validation of an mRNA vaccine platform expressing eGFP and development of a recombinant Streptococcus gordonii as live vaccine vector

Thesis project involved the development of an mRNA and a recombinant Streptococcus gordonii vaccine model. The mRNA vaccine was produced starting from the design of a DNA plasmid template encoding for the enhanced Green Fluorescent Protein (eGFP) (Chapter 2). The mRNA was transcribed, modified and complexed with a delivery system. The vaccine complex obtained was evaluated for the transfection efficiency and the protein expression in two mammalian cell lines: HeLa cells and C2C12 cells. The e...

Impact of Streptococcus pyogenes prophage Φ1207.3 on bacterial host response to stress conditions

The SOS response is an inducible system activated by some bacterial species in the presence of stressful conditions and extensive DNA damage. Streptococci lack a canonical SOS response, but an SOS-like response was reported in some species. The mef(A)-msr(D)-carrying prophage Ф1207.3 of Streptococcus pyogenes contains a DNA region, spanning from orf6 to orf11, showing homology to characterized streptococcal SOS-like cassettes. In the present thesis, the impact of prophage Φ1207.3 SOS-like cas...

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