The main focus of this PhD thesis is the research of the mechanisms of resistance to beta-lactam antibiotics in health-care associated multi-resistant gram-negative bacteria (mainly Enterobacteriaceae). In particular, a novel class D beta-lactamase with carbapenemase activity, named OXA-372 was characterized from a multi-resistant Citrobacter freundii isolated from hospital-wastewater. Other interesting isolates included in this work are the first NMCA-producing Enterobacter ludwigii, the first IMI-2-producing E. ludwigii and a VIM-1-producing Aeromonas caviae. Moreover a cfr-positive linezolid resistant isolate of MRSA was characterized. Multiple techniques of molecular biology were applied during this thesis including PFGE, MLST, Real Time PCR and Whole-Genome Sequencing. Part of this work included also the italian epidemiology of carbapenemase and ESBL in a large collection of Escherichia coli and Klebsiella pneumoniae isolated from 14 different centers in 2013.
Scheda prodotto non validato
Scheda prodotto in fase di analisi da parte dello staff di validazione
|Titolo:||Characterization of resistance determinants in clinical and environmental isolates of multi-resistant bacteria|
|Appare nelle tipologie:||8.1 Tesi Dottorato|
File in questo prodotto: