This chapter discusses the selective relaxation techniques. The selective excitation of single protons simplifies the nuclear relaxation pathway and can be performed by a variety of techniques. If the experimental conditions are such that the selective relaxation process of a nucleus involved in dipole-dipole interactions is described by the reported equations, simple and reliable methods of calculation of relaxation parameters from selective relaxation measurements can be used, and structural and/or dynamic information can be obtained. In molecular systems in which closely spaced resonances and short relaxation times are present, the DANTE excitation technique or the one-dimensional version of the NOESY experiment should be used. Following a single or multiple selective excitations, peak intensities from partially relaxed spectra can be measured; initial rates can be obtained provided that the experiment is correctly carried out and theoretical problems do not quench the dipolar relaxation strategy. The formation of intermolecular adducts can be followed by selective relaxation measurements. It has been shown that the ligand-receptor interaction induces large changes in the direct relaxation contributions of the proton signals of the ligand.

Niccolai, N., Rossi, C. (1989). Selective Relaxation Techniques. In Nuclear Magnetic Resonance Part A: Spectral Techniques and Dynamics (pp. 184-199). San Diego : Academic Press Inc. [10.1016/0076-6879(89)76012-2].

Selective Relaxation Techniques

Niccolai, Neri;Rossi, Claudio
1989-01-01

Abstract

This chapter discusses the selective relaxation techniques. The selective excitation of single protons simplifies the nuclear relaxation pathway and can be performed by a variety of techniques. If the experimental conditions are such that the selective relaxation process of a nucleus involved in dipole-dipole interactions is described by the reported equations, simple and reliable methods of calculation of relaxation parameters from selective relaxation measurements can be used, and structural and/or dynamic information can be obtained. In molecular systems in which closely spaced resonances and short relaxation times are present, the DANTE excitation technique or the one-dimensional version of the NOESY experiment should be used. Following a single or multiple selective excitations, peak intensities from partially relaxed spectra can be measured; initial rates can be obtained provided that the experiment is correctly carried out and theoretical problems do not quench the dipolar relaxation strategy. The formation of intermolecular adducts can be followed by selective relaxation measurements. It has been shown that the ligand-receptor interaction induces large changes in the direct relaxation contributions of the proton signals of the ligand.
1989
978-0-12-182077-0
Niccolai, N., Rossi, C. (1989). Selective Relaxation Techniques. In Nuclear Magnetic Resonance Part A: Spectral Techniques and Dynamics (pp. 184-199). San Diego : Academic Press Inc. [10.1016/0076-6879(89)76012-2].
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/34059
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo