The interaction between neurons and glia is pivotal for the development of chronic opioid tolerance. One of the most important mechanisms of cell-to-cell interaction is the Notch signaling pathway. In this chapter we propose a double-immunofluorescence method to observe and quantify the colocalization of Notch-1 and mu-opioid receptor (MOR-1), using both neuronal and astrocyte markers.
Borgonetti, V., Galeotti, N. (2021). Fluorescence colocalization analysis of cellular distribution of mor-1. In Santi M. Spampinato (a cura di), Methods in Molecular Biology (pp. 27-34). New York : Humana Press Inc. [10.1007/978-1-0716-0884-5_3].
Fluorescence colocalization analysis of cellular distribution of mor-1
Borgonetti V.;
2021-01-01
Abstract
The interaction between neurons and glia is pivotal for the development of chronic opioid tolerance. One of the most important mechanisms of cell-to-cell interaction is the Notch signaling pathway. In this chapter we propose a double-immunofluorescence method to observe and quantify the colocalization of Notch-1 and mu-opioid receptor (MOR-1), using both neuronal and astrocyte markers.
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https://hdl.handle.net/11365/1289085