In this study, we investigated the physicochemical and biological properties of naked and coated titania nanoparticles. The aim of this study was to verify the effect of the biopolymer coatings (hyaluronic acid and its biphosphonated derivative) and the role of protein adsorption from a cell culture medium on the citotoxicity of nanoparticles. Infrared spectroscopy (FT-IR) was used to investigate the interactions between the nanoparticles and the polysaccharides. The ζ potentials and the average hydrodynamic diameters of naked and coated nanoparticles dispersed in deionized water, medium with and without fetal bovine serum, were measured by means of dynamic light scattering (DLS). FT-IR and DLS measurements indicate that serum proteins are adsorbed on the NPs' surface. The biological tests show that naked and coated TiO(2) NPs do not induce an acute toxic effect on fibroblast cell cultures. This result shows that protein adsorption on NPs is an important factor in explaining the effect of NPs on cellular behavior.

Pasqui, D., Golini, L., Della Giovampaola, C., Atrei, A.M., Barbucci, R. (2011). Chemical and Biological Properties of Polysaccharides-Coated Titania Nanoparticles: The Key Role of Proteins. BIOMACROMOLECULES, 12(4), 1243-1249 [10.1021/bm101521e].

Chemical and Biological Properties of Polysaccharides-Coated Titania Nanoparticles: The Key Role of Proteins

Pasqui, Daniela;Golini, Lucia;Della Giovampaola, Cinzia;Atrei, Andrea Massimo;Barbucci, Rolando
2011-01-01

Abstract

In this study, we investigated the physicochemical and biological properties of naked and coated titania nanoparticles. The aim of this study was to verify the effect of the biopolymer coatings (hyaluronic acid and its biphosphonated derivative) and the role of protein adsorption from a cell culture medium on the citotoxicity of nanoparticles. Infrared spectroscopy (FT-IR) was used to investigate the interactions between the nanoparticles and the polysaccharides. The ζ potentials and the average hydrodynamic diameters of naked and coated nanoparticles dispersed in deionized water, medium with and without fetal bovine serum, were measured by means of dynamic light scattering (DLS). FT-IR and DLS measurements indicate that serum proteins are adsorbed on the NPs' surface. The biological tests show that naked and coated TiO(2) NPs do not induce an acute toxic effect on fibroblast cell cultures. This result shows that protein adsorption on NPs is an important factor in explaining the effect of NPs on cellular behavior.
2011
Pasqui, D., Golini, L., Della Giovampaola, C., Atrei, A.M., Barbucci, R. (2011). Chemical and Biological Properties of Polysaccharides-Coated Titania Nanoparticles: The Key Role of Proteins. BIOMACROMOLECULES, 12(4), 1243-1249 [10.1021/bm101521e].
File in questo prodotto:
File Dimensione Formato  
79.bm101521e.pdf

non disponibili

Tipologia: Post-print
Licenza: NON PUBBLICO - Accesso privato/ristretto
Dimensione 2.16 MB
Formato Adobe PDF
2.16 MB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/21311
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo