The identification of reproductive toxicants is a major scientific challenge for human health. We investigated the effects of a selected group of environmental polluting chemicals mostly provided with estrogenic activity on the human trophoblast cell lines BeWo and HTR-8/SVneo. Cells were exposed for 24 hours to various concentrations (from 0.1 pM to 1 mM) of atrazine (ATR), diethylstilbestrol (DES), para-nonylphenol (p-NP), resveratrol (RES) and 17 β-estradiol (E2) and assayed for cell viability and human beta- Chorionic Gonadotropin (β-hCG) secretion. Decrease of cell viability as respect to control, vehicle-treated, cultures was obtained for all chemicals in the concentration range of 1 μM – 1 mM in both cell types. A parallel decrease of β-hCG secretion was observed in BeWo cells, at 1 μM – 1 mM concentrations, with the only exception of ATR which caused an increase at concentrations up to 1 mM. β-hCG release was also unexpectedly inhibited by ATR, DES, p-NP and RES at non-toxic (pM-nM) concentrations These findings raise concern about the negative, potential effects of various environmental polluting chemicals on pregnancy success and fetal health.

Bechi, N., Sorda, G., Spagnoletti, A., Bhattacharjee, J., Vieira Ferro, E.A., De Freitas Barbosa, B., et al. (2013). Toxicity assessment on trophoblast cells for some environment polluting chemicals and 17β-estradiol. TOXICOLOGY IN VITRO, 27(3), 995-1000 [10.1016/j.tiv.2013.01.013].

Toxicity assessment on trophoblast cells for some environment polluting chemicals and 17β-estradiol

Bechi, N.;Sorda, G.;Spagnoletti, A.;Bhattacharjee, J.;Frosini, M.;Valoti, M.;Sgaragli, G.;Ricci, L.;Ietta, F.
2013-01-01

Abstract

The identification of reproductive toxicants is a major scientific challenge for human health. We investigated the effects of a selected group of environmental polluting chemicals mostly provided with estrogenic activity on the human trophoblast cell lines BeWo and HTR-8/SVneo. Cells were exposed for 24 hours to various concentrations (from 0.1 pM to 1 mM) of atrazine (ATR), diethylstilbestrol (DES), para-nonylphenol (p-NP), resveratrol (RES) and 17 β-estradiol (E2) and assayed for cell viability and human beta- Chorionic Gonadotropin (β-hCG) secretion. Decrease of cell viability as respect to control, vehicle-treated, cultures was obtained for all chemicals in the concentration range of 1 μM – 1 mM in both cell types. A parallel decrease of β-hCG secretion was observed in BeWo cells, at 1 μM – 1 mM concentrations, with the only exception of ATR which caused an increase at concentrations up to 1 mM. β-hCG release was also unexpectedly inhibited by ATR, DES, p-NP and RES at non-toxic (pM-nM) concentrations These findings raise concern about the negative, potential effects of various environmental polluting chemicals on pregnancy success and fetal health.
2013
Bechi, N., Sorda, G., Spagnoletti, A., Bhattacharjee, J., Vieira Ferro, E.A., De Freitas Barbosa, B., et al. (2013). Toxicity assessment on trophoblast cells for some environment polluting chemicals and 17β-estradiol. TOXICOLOGY IN VITRO, 27(3), 995-1000 [10.1016/j.tiv.2013.01.013].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/44375
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