In this paper, a method based on NMR measurements for studying the ability of ligands to interact with macromolecule surfaces is proposed. It is based on temperature-dependent NMR proton selective and non-selective spin lattice relaxation rate experiments and assumes fast chemical exchange conditions of the ligand between free and bound states. The results obtained appear able to identify DNA-psoralen complex formation. The use of this melhodology for monitoring non-covalent interaction of ligands and bipolymers is suggested.

Bonechi, C., Donati, A., Picchi, M.P., Rossi, C., Tiezzi, E. (1996). DNA-ligand interaction detected by proton-selective and non-selective spin-lattice relaxation rate analysis. COLLOIDS AND SURFACES. A, PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 115, 89-95.

DNA-ligand interaction detected by proton-selective and non-selective spin-lattice relaxation rate analysis

BONECHI, CLAUDIA;DONATI, ALESSANDRO;PICCHI, MARIA PIA;ROSSI, CLAUDIO;TIEZZI, ENZO
1996-01-01

Abstract

In this paper, a method based on NMR measurements for studying the ability of ligands to interact with macromolecule surfaces is proposed. It is based on temperature-dependent NMR proton selective and non-selective spin lattice relaxation rate experiments and assumes fast chemical exchange conditions of the ligand between free and bound states. The results obtained appear able to identify DNA-psoralen complex formation. The use of this melhodology for monitoring non-covalent interaction of ligands and bipolymers is suggested.
1996
Bonechi, C., Donati, A., Picchi, M.P., Rossi, C., Tiezzi, E. (1996). DNA-ligand interaction detected by proton-selective and non-selective spin-lattice relaxation rate analysis. COLLOIDS AND SURFACES. A, PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 115, 89-95.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/31966
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