The spectral behaviour of calcein, a water-soluble self-quenching molecule that is often used as a marker in biomedical analysis, can be considerably affected by the presence of surfactants. The aim of this work was to investigate the spectral properties of calcein under conditions that are particularly significant in liposome studies. For this purpose the fluorescence and absorbance of this dvc were determined in solutions of ionic and non-ionic surfactants (cetyltrimethylammonium bromide, sodium dodecyl sulphate and Triton X-100), at different concentrations below and above their critical micelle concentrations (c.m.c.) as well as in the presence of phospholipids in the form of liposomes and/or mixed micelles. Cationic surfactants induced changes in λmax, absorbance and fluorescence but these changes were less noticeable in the phospholipid dispersions. The anionic and the non-ionic surfactants induced mainly changes in fluorescence intensity. © 1994.

Memoli, A., Palermiti, L.G., Travagli, V., Alhaique, F. (1994). Effects of surfactants on the spectral behaviour of calcein. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 12(3), 307-312 [10.1016/0731-7085(94)90004-3].

Effects of surfactants on the spectral behaviour of calcein

Travagli, Valter;
1994-01-01

Abstract

The spectral behaviour of calcein, a water-soluble self-quenching molecule that is often used as a marker in biomedical analysis, can be considerably affected by the presence of surfactants. The aim of this work was to investigate the spectral properties of calcein under conditions that are particularly significant in liposome studies. For this purpose the fluorescence and absorbance of this dvc were determined in solutions of ionic and non-ionic surfactants (cetyltrimethylammonium bromide, sodium dodecyl sulphate and Triton X-100), at different concentrations below and above their critical micelle concentrations (c.m.c.) as well as in the presence of phospholipids in the form of liposomes and/or mixed micelles. Cationic surfactants induced changes in λmax, absorbance and fluorescence but these changes were less noticeable in the phospholipid dispersions. The anionic and the non-ionic surfactants induced mainly changes in fluorescence intensity. © 1994.
1994
Memoli, A., Palermiti, L.G., Travagli, V., Alhaique, F. (1994). Effects of surfactants on the spectral behaviour of calcein. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 12(3), 307-312 [10.1016/0731-7085(94)90004-3].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/29371
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