1. In the presence of MgATP and increasing amounts of added Ca2+, isolated liver microsomal vesicles accumulate approx. 10 nmol of Ca2+/mg of protein and buffer ambient free Ca2+ at increasing concentrations (0.22-10.9 μM). Under these experimental conditions, microsomal glucose-6-phosphatase activity is unaffected by the concentration of extravesicular free Ca2+. 2. Different levels of intravesicular Ca2+ were obtained by treating microsomes with the Ca2+ ionophore A23187 and by stimulating active microsomal Ca2+ accumulation with P1 (3 mM). In both instances, microsomal glucose-6-phosphatase activity is unaffected by the level of intravesicular Ca2+.

Fulceri, R., Bellomo, G., Gamberucci, A., Benedetti, A. (1991). Liver glucose-6-phosphatase activity is not modulated by physiological intracellular Ca2+ concentrations. BIOCHEMICAL JOURNAL, 275(3), 805-807 [10.1042/bj2750805].

Liver glucose-6-phosphatase activity is not modulated by physiological intracellular Ca2+ concentrations

Fulceri, R.;Gamberucci, A.;Benedetti, A.
1991-01-01

Abstract

1. In the presence of MgATP and increasing amounts of added Ca2+, isolated liver microsomal vesicles accumulate approx. 10 nmol of Ca2+/mg of protein and buffer ambient free Ca2+ at increasing concentrations (0.22-10.9 μM). Under these experimental conditions, microsomal glucose-6-phosphatase activity is unaffected by the concentration of extravesicular free Ca2+. 2. Different levels of intravesicular Ca2+ were obtained by treating microsomes with the Ca2+ ionophore A23187 and by stimulating active microsomal Ca2+ accumulation with P1 (3 mM). In both instances, microsomal glucose-6-phosphatase activity is unaffected by the level of intravesicular Ca2+.
1991
Fulceri, R., Bellomo, G., Gamberucci, A., Benedetti, A. (1991). Liver glucose-6-phosphatase activity is not modulated by physiological intracellular Ca2+ concentrations. BIOCHEMICAL JOURNAL, 275(3), 805-807 [10.1042/bj2750805].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/28342
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