Experiments assessed whether long term exposure to 50 Hz pulsed electromagnetic fields with a peak magnetic field of 3 mT can alter the dynamics of intracellular calcium in human astrocytoma U-373 MG cells. Pretreatment of cells with 1.2 μM substance P significantly increased the [Ca2+]i. The same effect was also observed when [Ca2+]i was evaluated in the presence of 20 mM caffeine. After exposure to electromagnetic fields the basal [Ca2+]i levels increased significantly from 143 ± 46 nM to 278 ± 125 nM. The increase was also evident after caffeine addition, but in cells treated with substance P and substance P + caffeine we observed a [Ca2+]i decrease after exposure. When we substituted calcium-free medium for normal medium immediately before the [Ca2+]i measurements, the [Ca2+]i was similar to that measured in the presence of Ca2+. In this case, after EMFs exposure of cells treated with substance P, the [Ca2+]i, measured without and with addition of caffeine, declined from 824 ± 425 to 38 ± 13 nM and from 1369 ± 700 to 11 ± 4 nM, respectively, indicating that electromagnetic fields act either on intracellular Ca2+ stores or on the plasma membrane. Moreover the electromagnetic fields that affected [Ca2+]i did not cause cell proliferation or cell death and the proliferation indexes remained unchanged after exposure. © 2001 Wiley-Liss, Inc.

Pessina, G.P., Aldinucci, C., Palmi, M., Sgaragli, G., Benocci, A., Meini, A., et al. (2001). Pulsed electromagnetic fields affect the intracellular calcium concentrations in human astrocytoma cells. BIOELECTROMAGNETICS, 22(7), 503-510 [10.1002/bem.79].

Pulsed electromagnetic fields affect the intracellular calcium concentrations in human astrocytoma cells

Pessina, G. P.;Aldinucci, Carlo;Palmi, Mitri;Sgaragli, G.;Meini, A.;Pessina, Federica
2001-01-01

Abstract

Experiments assessed whether long term exposure to 50 Hz pulsed electromagnetic fields with a peak magnetic field of 3 mT can alter the dynamics of intracellular calcium in human astrocytoma U-373 MG cells. Pretreatment of cells with 1.2 μM substance P significantly increased the [Ca2+]i. The same effect was also observed when [Ca2+]i was evaluated in the presence of 20 mM caffeine. After exposure to electromagnetic fields the basal [Ca2+]i levels increased significantly from 143 ± 46 nM to 278 ± 125 nM. The increase was also evident after caffeine addition, but in cells treated with substance P and substance P + caffeine we observed a [Ca2+]i decrease after exposure. When we substituted calcium-free medium for normal medium immediately before the [Ca2+]i measurements, the [Ca2+]i was similar to that measured in the presence of Ca2+. In this case, after EMFs exposure of cells treated with substance P, the [Ca2+]i, measured without and with addition of caffeine, declined from 824 ± 425 to 38 ± 13 nM and from 1369 ± 700 to 11 ± 4 nM, respectively, indicating that electromagnetic fields act either on intracellular Ca2+ stores or on the plasma membrane. Moreover the electromagnetic fields that affected [Ca2+]i did not cause cell proliferation or cell death and the proliferation indexes remained unchanged after exposure. © 2001 Wiley-Liss, Inc.
2001
Pessina, G.P., Aldinucci, C., Palmi, M., Sgaragli, G., Benocci, A., Meini, A., et al. (2001). Pulsed electromagnetic fields affect the intracellular calcium concentrations in human astrocytoma cells. BIOELECTROMAGNETICS, 22(7), 503-510 [10.1002/bem.79].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/21532
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