Following disruption of the nerve supply, extensor digitorum longus (EDL) and soleus (SOL) muscles in rats are known to exhibit alterations in excitation-contraction coupling. After total RNA isolation from the denervated and the contralateral control muscles performed at 25 and 50 days following denervation, RNase protection assays were carried out with four cDNA probes specific for the skeletal and cardiac isoforms of both the DHPR alpha 1-subunit and the RyR. Longterm denervation increased the expression of the mRNA for skeletal DHPR and skeletal RyR in SOL muscle, but it also significantly increased the expression of the mRNA for the cardiac isoform of the DHPR alpha 1 subunit in EDL muscle.

Péréon, Y., Sorrentino, V., Dettbarn, C., Noireaud, J., Palade, P. (1997). Dihydropyridine receptor and ryanodine receptor gene expression in long-term denervated rat muscles. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 240(3), 612-617 [10.1006/bbrc.1997.7712].

Dihydropyridine receptor and ryanodine receptor gene expression in long-term denervated rat muscles

Sorrentino, V.;
1997-01-01

Abstract

Following disruption of the nerve supply, extensor digitorum longus (EDL) and soleus (SOL) muscles in rats are known to exhibit alterations in excitation-contraction coupling. After total RNA isolation from the denervated and the contralateral control muscles performed at 25 and 50 days following denervation, RNase protection assays were carried out with four cDNA probes specific for the skeletal and cardiac isoforms of both the DHPR alpha 1-subunit and the RyR. Longterm denervation increased the expression of the mRNA for skeletal DHPR and skeletal RyR in SOL muscle, but it also significantly increased the expression of the mRNA for the cardiac isoform of the DHPR alpha 1 subunit in EDL muscle.
1997
Péréon, Y., Sorrentino, V., Dettbarn, C., Noireaud, J., Palade, P. (1997). Dihydropyridine receptor and ryanodine receptor gene expression in long-term denervated rat muscles. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 240(3), 612-617 [10.1006/bbrc.1997.7712].
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11365/20822
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo